PMSF Cas: 329-98-6 98.0% ọcha crystalline ntụ ntụ Phenylmethanesulfonyl fluoride (PMSF)
| Nọmba katalọgụ | XD90250 |
| Aha ngwaahịa | Phenylmethanesulfonyl fluoride (PMSF) |
| CAS | 329-98-6 |
| Usoro ihe omimi | C7H7FO2S |
| Ibu molekụla | 174.1927 |
| Nkọwa nchekwa | Ambient |
| Koodu tarifu akwadoro | 29049900 |
Nkọwapụta ngwaahịa
| Nyocha | ≥98.0% HPLC |
| Ọdịdị | ọcha crystalline ntụ ntụ |
PMSF bụ serine/cysteine protease inhibitor na-adịghị agbanwe agbanwe nke a na-ejikarị na nkwadebe nke cell lysates.
Ọmụmụ in vitro: PMSF (2 mM) na-egbochi mkpokọta inositol phosphate nke carbachol na-akpali naanị 15%-19% na ọnụnọ Li +.Mgbochi nke ntụgharị phosphoinotide site na PMSF bụ n'ihi otu usoro ma ọ bụ karịa na-eso nkwụsị nke phosphoinotides [1].PMSF na-egbochi acylation nke inositol residues nke GPI intermediates n'ime ọbara nke T. brucei.PMSF na-egbochi nhazi glycolipid C mana ọ bụghị imegharị acid fatty na vitro.PMSF na-egbochi mgbakwunye GPI acylation na ethanolamine phosphatase na procyclic trypanosomes, mana ọ bụghị na sel Hela [2].
Na ọmụmụ vivo: PMSF (0.1 mL / 10 g b.wt, ip) mepụtara antinociception, dị ka egosipụtara site na mmụba na-anabata dose na% MPE na nyocha latency ọdụ ọdụ, mana ọ naghị emepụta ihe mgbochi moto na-anabata nke ọma.Ụmụ oke natara intraperitoneal injection nke PMSF gosipụtara mmetụta cannabinoid gụnyere antinociception, hypothermia na immobility na ED50 ụkpụrụ nke 86, 224 na 206 mg / kg, n'otu n'otu.PMSF (30 mg / kg) pretreatment na-eme ka mmetụta nke anandamide na nzaghachi ọdụ ọdụ (antinociception), ọrụ locomotor na mmegharị site na 5-fold, 10-fold and 8-fold, n'otu n'otu [3].
Nnwale anụmanụ: A na-eji ụmụ oke ICR nwoke na-eru 18 ruo 25 g na nyocha.A gbazere PMSF na mmanụ sesame ma nye ya intraperitoneal n'ogo nke 0.1 mL / 10 g b.wt.Na-enye PMSF oge niile nkeji iri tupu anadamide intravenous ma ọ bụ ntụtụ ụgbọ ala.A kpọbatara ụmụ oke n'ọnụ ụlọ nyocha n'otu abalị na-enweghị nkwụsị nke nri ma ọ bụ mmiri.Na-eso anandamide intravenous ma ọ bụ nchịkwa ụgbọ ala, a na-enyocha anụmanụ ọ bụla dị ka ndị a: 5 nkeji maka nzaghachi ọdụ-flick (antinociceptive) nzaghachi na 5 ruo 15 nkeji maka ọrụ mberede (moto);ma ọ bụ nkeji 5 maka okpomoku isi (rectal) na mgbanaka Immobilization (catalepsy) maka nkeji 5 ruo 10.
