Ngwaahịa

Okwu mmalite: BSA bụ otu n'ime protein ndị a na-ejikarị eme ihe na ụlọ nyocha biochemical, enwere ike ileghara ịdị mkpa ya anya na nnwale n'ihi na ọ na-adịkarị na nke nkịtị.Serum albumin nke Bovine (BSA), nke a makwaara dị ka akụkụ nke ise, bụ globulin na serum bovine nwere 583 amino acid residues, nke nwere ịdị arọ molekul nke 66.430kDa yana ebe isoelectric nke 4.7.BSA nwere ngwa dị iche iche na nnwale biochemical, dị ka onye na-egbochi ihe na ebe ọdịda anyanwụ.

Ngwa: Bovine Serum Albumin (BSA), nke a makwaara dị ka akụkụ nke ise, bụ globulin na serum bovine, nke nwere 607 amino acid residues, ma nwee ọtụtụ ngwa na nyocha biochemical.A na-ejikarị bovine serum albumin eme ihe dị ka ihe nkwụsi ike na ngwọta nchekwa na mmeghachi omume mmeghachi omume nke mgbochi enzymes ma ọ bụ gbanwetụrụ iji mee ka ọrụ enzyme kwụsie ike ma gbochie ire enzyme na mgbasa ozi na-abụghị nke akọwapụtara.

Arụ ọrụ: A na-ejikarị BSA eme ihe dị ka ihe nkwụsi ike na ngwọta nchekwa na mmeghachi omume mmeghachi omume nke mmachi enzymes ma ọ bụ gbanwetụrụ enzymes, n'ihi na ụfọdụ enzymes anaghị akwụsi ike ma ọ bụ nwee obere ọrụ na obere mkpokọta.Mgbe ịgbakwunye BSA, ọ nwere ike ịrụ ọrụ nke "nchekwa" ma ọ bụ "bu", na ọrụ nke ọtụtụ enzymes nwere ike imeziwanye nke ọma mgbe ịgbakwunye BSA.Enzymes nke na-achọghị mgbakwunye nke BSA anaghị emetụtakarị site na mgbakwunye BSA.Maka ọtụtụ mkpụrụ DNA, BSCA nwere ike ime ka mgbari mgbaze ahụ zuo oke, ma nwee ike nweta igbutu ugboro ugboro.Na 37 Celsius C, mgbe mmeghachi omume mgbaze gafere 1 h, BSA nwere ike ime ka enzyme ahụ kwụsie ike, n'ihi na na nzaghachi mmeghachi omume na-enweghị BSA, ọtụtụ enzymes mgbochi nwere ike ịdị ndụ na 37 ° C maka 10 ~ 20min ma ọ bụ ọbụna obere oge..N'ụzọ dị iche, BSA nwere ike jikọta ion metal na kemịkalụ ndị ọzọ na nchekwa ma ọ bụ mkpụrụ DNA nke na-egbochi ọrụ mmachi endonucleases.

Na-eji: Standard ọkwa bovine serum albumin (BSA, StandardGrade), nke nwere ike izute mkpa nke ọtụtụ oge nnwale, dị ka immunoblocking agents, anụ ahụ cell (microbial anụmanụ na ụmụ ahụhụ, wdg) omenala nri na omenala components, protein / enzyme stabilization. reagents na ụkpụrụ quantification protein.Nchọpụta ọkwa bovine serum albumin (BSA, DiagnosticGrade) nwere ike gboo ọtụtụ mkpa nnwale oge niile, dị ka ihe mgbochi mgbochi, protein/enzyme stabilizer, diluent, ebu na ọkọlọtọ protein.Tụkwasị na nke a, a pụkwara iji ya mee ihe maka immunoassays, omenala cell na hybridization nnwale nke chọrọ nnukwu uche.