Ngwaahịa

LecA (PA-IL) bụ lectin cytotoxic na adhesin mepụtara site na Pseudomonas aeruginosa nke na-ejikọta hydrophobic galactosides na nnukwu nkọwa na mmekọrịta.Site n'iji ntughari ntụgharị lecA-egfp na nyocha immunoblot nke biofilm extracellular matrix, anyị na-egosi na a na-egosipụta lecA na sel ndị toro eto.N'ime nyocha biofilm static na ma polystyrene na igwe anaghị agba nchara, omimi biofilm na mkpuchi elu belatara site na ngbanwe nke lecA wee kwalite na LecA na-emepụta ihe karịrị PAO-P47.Mkpuchi elu biofilm site n'aka nne na nna, PAO-P47 mana ọbụghị lecA mutant na Kupọns nchara ka egbochikwara uto na ọnụnọ nke isopropyl-beta-D-thiogalactoside (IPTG) ma ọ bụ p-nitrophenyl-alpha-D-galactoside ( NPG).Ọzọkwa, ụdị biofilms ndị tozuru oke na-etolite na enweghị galactosides hydrophobic nwere ike gbasasịa site na mgbakwunye nke IPTG.N'ụzọ dị iche, mgbakwunye p-nitrophenyl-alpha-L-fucose (NPF) nke nwere mmekọrịta dị elu maka P. aeruginosa LecB (PA-IIL) lecti n enweghị mmetụta na nhazi biofilm ma ọ bụ gbasaa.Planktonic uto nke P. aeruginosa PAO1 emetụtaghị ọnụnọ nke IPTG, NPG ma ọ bụ NPF, ma ọ bụ nsogbu ahụ enweghị ike iji sugars ndị a dị ka isi iyi carbon, na-atụ aro na mmetụta ndị a hụrụ na nhazi biofilm bụ n'ihi nkwụsị asọmpi nke LecA-ligand. njide.E nwetakwara nsonaazụ yiri nke ahụ maka biofilms toro n'okpuru ọnọdụ ịgba ọsọ siri ike na Kupọns ígwè, na-atụ aro na LecA na-enye aka na P. aeruginosa biofilm architecture n'okpuru ọnọdụ gburugburu ebe obibi dị iche iche.

Otu alfa-L-fucosidase (EC 3.2.1.51) nwere ike ịhapụ t-fucosyl residue si n'akụkụ akụkụ nke xyloglucan oligosaccharides achọpụtara na epupụta nke Arabidopsis osisi.Ọzọkwa, a sachara alfa-L-fucosidase nke nwere ụdị mkpụrụ osisi a dị ọcha site na akwụkwọ kabeeji (Brassica oleracea) iji nye otu eriri na SDS-PAGE.E nwetara usoro peptide abụọ site na eriri protein a, a na-ejikwa ha chọpụta mkpụrụ ndụ Arabidopsis maka alfa-fucosidase nke anyị na-atụ aro ịkpọ AtFXG1.Na mgbakwunye, a chọpụtakwara mkpụrụ ndụ Arabidopsis nwere homology nwere alfa-L-fucosidases amaara, anyị tụrụ aro ịkpọ ya dị ka AtFUC1.Ma AtFXG1 na ATFUC1 ka egosipụtara n'ụzọ dị iche iche na sel pastoris Pichia na ihe omume alfa-L-fucosidase zoro ezo na usoro omenala.Alfa-L-fucosidase nke AtFXG1 debere na-arụ ọrụ megide oligosaccharides sitere na xyloglucan XXFG yana megide 2'-fucosyl-lactitol mana ọ bụghị megide p-nitrophenyl-alpha-L-fucopyranoside.Agbanyeghị, AtFUC1 heterologously kwupụtara na-arụ ọrụ naanị megide 2'-fucosyl-lactitol.Ya mere, nke mbụ ga-ejikọta ya na metabolism xyloglucan.