Ngwaahịa

Mgbochi nke β-carbonic anhydrases (CAs, EC 4.2.1.1) sitere na fungi pathogenic Cryptococcus neoformans (Can2) na Candida albicans (Nce103) na usoro nke 25 branched aliphatic na aromatic carboxylates enyochala.Agụnyere isoforms mmadụ hCA I na II n'ọmụmụ ihe maka ntụnyere.Carboxylates aliphatic bụ ihe mgbochi hCA I na II millimolar na obere micromolar/submicromolar β-CA inhibitors.Carboxylates aromatic bụ ndị na-egbochi micromolar nke enzymes anọ mana ụfọdụ n'ime ha gosipụtara obere ọrụ nanomolar megide enzymes pathogenic fungal.4-Hydroxy- na 4-methoxy-benzoate gbochiri Can2 na K (I) s nke 9.5-9.9 nM.methyl esters, hydroxamates, hydrazides na carboxamides nke ụfọdụ n'ime usoro ndị a bụkwa ndị na-egbochi α- na β-CA dị irè ebe a.

Parabens so na ihe nchekwa ndị a na-ejikarị egbochi uto microbial ma gbatịa ndụ nchekwa nke ngwaahịa dị iche iche.Ebumnuche nke ọmụmụ ihe a bụ iji nweta nghọta na metabolism nke parabens na mkpụrụ ndụ cancer ara (MCF7) ebe ọ bụ na ha egosipụtawo ọrụ estrogenic n'ebe mkpụrụ ndụ ndị a nọ ma chọpụta na anụ ahụ cancer ara.Achọpụtara nsi nke parabens na sel MCF7 site na iji nyocha MTT.A na-enyocha hydrolysis nke methyl-, butyl na benzyl-paraben na p-hydroxybenzoic acid na mkpụrụ ndụ MCF7 omenala yana na homogenates cellular.A mụrụ Glucuronidation na sulfoconjugation na MCF7 homogenates, na HPLC nyochara parabens.E gosiputara Methyl-paraben dị obere nsí karịa butyl na benzyl-paraben.Parabens kwụsiri ike na MCF7 homogenates ebe p-nitrophenyl acetate, ụdị mkpụrụ, mere hydrolysis.MCF7 cell homogenates ekwupụtaghị glucuronidation na sulfoconjugation omume na parabens.Nkwụsi ike dị elu nke parabens nwere ike ịkọwa mkpokọta ha na anụ ahụ ọrịa kansa ara.