Ngwaahịa

A na-emepụta immunosensor electrochemiluminescence na-enweghị akara (ECL) maka nchọpụta ultrasensitive na ngwa ngwa nke nje pathogenic mmiri Vibrio parahaemolyticus (VP) na mmiri mmiri na ihe oriri na-edozi ahụ dabere na graphene oxide multi-functionalized, nke a kwadebere na N- (4-aminobutyl) -N. -ethylisoluminol (ABEI) na VP mgbochi (mgbochi VP) n'otu oge na-adịghị emegharị n'elu magnetik graphene oxide (nanoFe3O4 @ GO).ABEI na mgbochi VP mere dị ka electrochemiluminophore na ngwaọrụ njide maka VP n'otu n'otu.Ezi conductivity na abụọ akụkụ Ọdịdị nke nanoFe3O4 @ GO nyeere ndị niile ABEI, immobilized na GO, electrochemically arụ ọrụ ma si otú mma nchọpụta uche.N'okpuru ọnọdụ kachasị mma, ike ECL belatara site na mmụba logarithmic nke VP na oke nke 10-10 (8) CFU / mL, yana njedebe nchọpụta nke 5CFU/mL maka mmiri mmiri na 5CFU/g maka nri mmiri.Nke a immunosensor ECL gosipụtara nkọwapụta dị elu, nkwụsi ike na nrụpụta maka nchọpụta VP.Na mgbakwunye, e jiriwo immunosensor ECL nke ọma mee ihe iji chọpụta ntinye nke VP na mmiri mmiri na nri mmiri ngwa ngwa, na mgbake nke 94.4-112.0% na RSD 4.1-11.7%.Ya mere, immunosensor mepere emepe na-egosi nnukwu atụmanya maka ngwa bara uru.