Ngwaahịa

Yiri nje virus RNA ndị ọzọ dị mma, nje virus tombus na-emegharị ya site na membran-bound viral replicase complex (VRC).VRC nwere p92 nje nwere koodu RNA-based RNA polymerase (RdRp), viral p33 RNA chaperone, na ọtụtụ protein ndị ejikọtara ọnụ.Ka ọ bụrụ RdRp na-arụ ọrụ mgbe ntụgharị ya gasịrị, a ga-etinye protein mmegharị p92 na VRC, na-esote ya.Anyị egosila na mbụ n'ihe nyocha dabere na ihe yist na-enweghị cell na ịgbalite nje stunt Tomato bushy stunt (TBSV) RdRp chọrọ ihe (s) nnabata soluble.N'edemede a, anyị na-achọpụta protein okpomọkụ ujo cell 70 (Hsp70) dị ka ihe ejikọtara ọnụ nke achọrọ maka ịgbalite TBSV RdRp N-n'ikpeazụ truncated.Na mgbakwunye, nkwụsị nke ọrụ Hsp70 sitere na obere-molecule na-egbochi njikọ RNA site na tombusvirus RdRp in vitro.Ọzọkwa, anyị na-egosi na phospholipids na-anọpụ iche, ya bụ, phosphatidylethanolamine (PE) na phosphatidylcholine (PC), na-eme ka RdRp rụọ ọrụ na vitro.N'ụzọ dị iche, phosphatidylglycerol (PG) na-egosi mmetụta mgbochi siri ike na nke kachasị na in vitro RdRp ịgbalite.Anyị na-egosipụtakwa na PE na PC na-akpali mmekọrịta RdRp-viral plus-strand RNA [(+) RNA], ebe PG na-egbochi njikọ nke viral RNA na RdRp.Dabere na ọrụ na-akpali akpali vesos inhibitory nke dị iche iche phospholipids na tombusvirus RdRp activation, anyị na-atụ aro na a ga-eji ihe mejupụtara nke lipid membranes subcellular ezubere iche iji hazie mgbakọ VRC ọhụrụ n'oge ọrịa nje. (RdRp), bụ onye na-ahụ maka ịmepụta nje virus RNA n'ime mkpụrụ ndụ nje nke ọtụtụ nje RNA dị mma, anaghị arụ ọrụ na mbụ.Usoro a nwere ike ịzenarị njikọ RNA viral na cytosol nke ga-eduga ngwa ngwa n'iwebata nzaghachi mgbochi nje na cell RNA.N'oge mgbakọ nke membran-bound replicase complex, RdRp viral na-arụ ọrụ site na usoro aghọtachaghị nke na-eme ka RdRp nwee ike ịmekọrịta RNA.Site na iji TBSV RdRp, anyị na-egosi na cellular Hsp70 chaperone na phospholipids na-anọpụ iche na-eme ka RdRp rụọ ọrụ na vitro.N'ụzọ dị iche, phosphatidylglycerol (PG) nwere mmetụta mgbochi kachasị na in vitro RdRp activation na RdRp-viral RNA mmekọrịta, na-atụ aro na microdomain membranous gbara RdRp gburugburu na-emetụta ike ya maka njikọ RNA.Yabụ, ịgbalite RdRp viral nwere ike dabere n'ọtụtụ akụrụngwa nnabata na sel ndị butere ọrịa.