Ngwaahịa

Nyocha nke ihe omume phosphorylation proteome ka bụ nnukwu ihe ịma aka nyocha n'ihi nnukwu mgbagwoju anya nke netwọk phosphorylation protein.N'ime ọrụ a, anyị na-enyocha mmeju nke Lys-N, Lys-C, na trypsin n'ihe gbasara ikike ha nwere itinye aka na nyocha zuru ụwa ọnụ nke phosphoproteome.A nụchara anụcha nke mgbanwe mgbanwe cation siri ike nke pH dị ala iji kewaa nke ọma N-terminally acetylated, phosphorylated, na peptides na-abụghị nke emezigharịrị.Enwere ike ịmata ngụkọta nke 5036 phosphopeptides na-adịghị akwụ ụgwọ site na ọnụọgụ nchọpụta ụgha nke <1% sitere na 1 mg nke protein site na iji ngwakọta nke enzymes atọ.Ihe omuma anyi gosiputara na nchikota n'etiti data phosphopeptide nke emeputara na protease di iche iche di obere, ebe nchikota n'etiti uzo data tryptic abuo abuo mere ka achoputara dika opekata mpe 4.N'ụzọ dị otu a, iji Lys-N na trypsin yiri ya mere ka ọnụọgụ phosphopeptides achọpụtara 72% dị ka atụnyere d na trypsin naanị, ebe nnwale nnwale nke trypsin butere naanị 25%.Ya mere, mgbe anyị na-elekwasị anya naanị na trypsin na data Lys-N, anyị chọpụtara 4671 phosphopeptides na-adịghị arụ ọrụ.Ntụle ọzọ nke saịtị ndị ahụ achọpụtara gosiri na ihe ndekọ data Lys-N na trypsin na-eme ka ọ baa ọgaranya n'ụdị phosphorylation dị iche iche, na-egosikwa na ụzọ multiprotease bara ezigbo uru na nyocha phosphoproteome.