X-GAL CAS: 7240-90-6 98% Ọcha na-acha ọcha kristal ntụ ntụ
| Nọmba katalọgụ | XD90008 |
| Aha ngwaahịa | X-Gal (5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside) |
| CAS | 7240-90-6 |
| Usoro ihe omimi | C14H15BrClNO6 |
| Ibu molekụla | 408.63 |
| Nkọwa nchekwa | -2 ruo -6 Celsius |
| Koodu tarifu akwadoro | 29400000 |
Nkọwapụta ngwaahịa
| Ọdịdị nke Ngwọta | Ngwọta edo edo doro anya, enweghị agba na-enweghị isi (50mg/ml na DMF:MeOH, 1:1) |
| Ntụgharị anya pụrụ iche | -61.5 +/- 1 |
| Ọdịdị | Uzuzu crystalline ọcha ruo na-acha ọcha |
| HPLC dị ọcha | nkeji 99% |
| Solubility (5% na DMF) | Soluble (5% w/v,DMF) |
| Mmiri KF | kacha 1% |
| Assay (HPLC na Anhydrous ndabere) | min 98% w/w |
Ojiji nke X-gal
X-gal (nke a na-akpọkwa BCIG maka 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside) bụ ngwakọta organic nke nwere galactose jikọtara na indole dochie anya.Ọ bụ Jerome Horwitz na ndị na-arụkọ ọrụ mebere ogige ahụ na 1964. A na-ebelatakarị aha kemịkalụ ka ọ bụrụ nke na-ezighi ezi mana ọ dịkwa obere nkebi ahịrịokwu dị ka bromochloroindoxyl galactoside.X sitere na indoxyl nwere ike ịbụ isi mmalite X na mkpịsị X-gal.A na-ejikarị X-gal eme ihe na ihe ndị dị ndụ ndụ iji nwalee ọnụnọ nke enzyme, β-galactosidase, n'ebe ọ na-ebubu ya, β-galactoside.A na-ejikwa ya chọpụta ọrụ nke enzyme a na histochemistry na bacteriology.X-gal bụ otu n'ime ọtụtụ indoxyl glycosides na esters nke na-emepụta ogige na-acha anụnụ anụnụ na-enweghị ike ime nke yiri indigo dye n'ihi enzyme-catalyzed hydrolysis.
X-gal bụ analọg nke lactose, yabụ enwere ike mebie ya site na β-galactosidase enzyme nke na-ekewa njikọ β-glycosidic na D-lactose.X-gal, mgbe β-galactosidase kewara ya, na-amịpụta galactose na 5-bromo-4-chloro-3-hydroxyindole - 1. Nke ikpeazụ ahụ na-adaba n'atụghị egwu ma bụrụ oxidized n'ime 5,5'-dibromo-4,4'-dichloro. -indigo - 2, ngwaahịa na-acha anụnụ anụnụ nke na-adịghị edozi.X-gal n'onwe ya enweghị agba, yabụ enwere ike iji ọnụnọ ngwaahịa na-acha anụnụ anụnụ mee nnwale maka ọnụnọ β-galactosidase na-arụ ọrụ.Nke a na-enyekwa ohere maka nje β-galactosidase (nke a na-akpọ lacZ) dị ka onye na-ede akụkọ na ngwa dị iche iche.
Na nyocha ngwakọ abụọ, β-galactosidase nwere ike iji dị ka onye nta akụkọ iji chọpụta protein ndị na-emekọrịta ihe.Na usoro a, enwere ike nyochaa ụlọ akwụkwọ genome maka mmekọrịta protein site na iji yist ma ọ bụ usoro nje.Ebe enwere mmekọrịta na-aga nke ọma n'etiti protein ndị a na-enyocha, ọ ga-ebute njikọ nke ngalaba ọrụ na onye nkwalite.Ọ bụrụ na onye na-akwalite ahụ jikọtara ya na mkpụrụ ndụ lacZ, mmepụta nke β-galactosidase, nke na-eme ka e nwee ebe ndị na-acha anụnụ anụnụ na-acha anụnụ anụnụ na ọnụnọ X-gal, ga-egosi na mmekọrịta dị n'etiti protein na-aga nke ọma.Usoro a nwere ike ịbụ naanị n'ọba akwụkwọ nyocha nke na-erughị gburugburu 106. Nkwụcha nke X-gal na-aga nke ọma na-emepụtakwa ísì ọjọọ nke ọma n'ihi mgbanwe nke indole.
Ebe X-gal n'onwe ya enweghị agba, enwere ike iji ọnụnọ ngwaahịa na-acha anụnụ anụnụ mee nnwale maka ọnụnọ β-galactosidase na-arụ ọrụ.
Nchọpụta dị mfe a nke enzyme na-arụ ọrụ na-enye ohere ka mkpụrụ ndụ ihe nketa βgalactosidase (gene lacZ) mee ihe dị ka mkpụrụ ndụ onye na-ede akụkọ na ngwa dị iche iche.
