Streptomycin sulfate CAS: 3810-74-0 ntụ ntụ ọcha
| Nọmba katalọgụ | XD90361 |
| Aha ngwaahịa | Streptomycin sulfate |
| CAS | 3810-74-0 |
| Usoro ihe omimi | (C21H39N7O12)2·3H2SO4 |
| Ibu molekụla | 1457.39 |
| Nkọwa nchekwa | 2 ruo 8 Celsius C |
| Koodu tarifu akwadoro | 29412080 |
Nkọwapụta ngwaahịa
| Ọkwa | USP36/EP8.0 |
| pH | 4.5-7.0 |
| Ọnwụ na ihicha | ≤7.0% |
| Nyocha | ≥720IU/MG |
| Sulfate | 18.0 ~ 21.5% |
| Ọdịdị | Ọcha ọcha ma ọ bụ ihe fọrọ nke nta ka ọ bụrụ ntụ ntụ, na-agbaze nke ukwuu n'ime mmiri, ọ fọrọ nke nta ka ọ ghara ịsacha ya na ethanol. |
| Methanol | ≥0.3% |
| Streptomycin B | ≤3.0% |
| Ule agba | ≥90.0% |
| Sulfuric acid | ≤1.0% |
| Ọdịnaya sodium sulfite gbadoro anya | ≤0.4% |
Ọ bụ ezie na etiology nke intervertebral disc degeneration aghọtachaghị nke ọma, otu ụzọ iji gbochie usoro a nwere ike ịbụ igbochi apoptosis.N'ime ọmụmụ ihe ugbu a, a na-enyocha mmetụta anti-apoptotic nke carboxymethylated chitosan (CMCS) na sel nucleus pulposus (NP) na ebumnuche iji kwalite ndụ diski diski.E kewapụrụ mkpụrụ ndụ NP oke ma mepụta ya na vitro, na hydrogen peroxide (H2O2) ka ejiri rụọ ụdị apoptosis cell NP.A tụlere ịdị ndụ cell site na iji ngwa ngụkọ cell-8.Achọpụtara nha nke mkpụrụ ndụ apoptotic site na annexin V-fluorescein isothiocyanate (FITC) na propidium iodide (PI) nyocha ntụpọ okpukpu abụọ, na ntụpọ Hoechst 33342 hụrụ morphology ahụ.A tụlere ike nke mitochondrial akpụkpọ anụ nke mkpụrụ ndụ NP site na ntụpọ rhodamine 123 fluorescence.A na-eme ntụgharị ntụgharị (RT)-quantitative polymerase chain reaction (qPCR) iji tụọ ọkwa mRNA nke inducible nitric oxide synthase (iNOS), caspase-3, B-cell lymphoma (Bcl) -2, ụdị II collagen na aggrecan.Emere nyocha nke Western blot iji chọpụta ọkwa protein nke iNOS na Bcl-2.Mgbakwunye V-FITC/PI na Hoechst 33342 nsonaazụ ntụpọ gosiri na CMCS nwere ike igbochi mkpụrụ ndụ NP site na apoptosis n'ụzọ dabere na dose.Rhodamine 123 nchacha mere ka o doo anya na CMCS belatara mmebi nke akpụkpọ anụ mitochondrial dị na mkpụrụ ndụ NP H2O2.Achọpụtara caspase-3 belatara yana mmụba Bcl-2 n'ime sel NP CMCS gwọchara site na nyocha RT‑qPCR na ọdịda anyanwụ blot.CMCS kwalitekwara mmụba na nzuzo nke ụdị II collagen na aggrecan na mkpụrụ ndụ NP H2O2.E gosipụtara CMCS ka ọ dị irè n'igbochi ọnwụ mkpụrụ ndụ apoptotic na vitro, na-egosipụta uru ndị nwere ike ime na usoro ọgwụgwọ a na-achịkwa mmebi diski.
