PIPES Cas: 5625-37-6 White crystalline ntụ ntụ 99% ABTS DIAMMONIUM SALT ULTRA PURE GRADE
| Nọmba katalọgụ | XD90117 |
| Aha ngwaahịa | PIPES (Piperazine-1,4-bis (2-ethanesulfonic acid)) |
| CAS | 5625-37-6 |
| Usoro ihe omimi | C8H18N2O6S2 |
| Ibu molekụla | 302.37 |
| Nkọwa nchekwa | Ambient |
| Koodu tarifu akwadoro | 2933599 |
Nkọwapụta ngwaahịa
| Ọla dị arọ | <5ppm |
| Ọnwụ na ihicha | <1.0% |
| Solubility | Ihe ngwọta doro anya, enweghị agba (5% 1N NaOH) |
| Nyocha | 99-101% |
| Ọdịdị | White crystalline ntụ ntụ |
A na-ejikarị PIPES [piperazine-N,N'-bis(2-ethanesulfonic acid)] dị ka onye na-echekwa ihe na biochemistry.Ọ bụ ihe nchekwa ethanesulfonic acid nke Good et al mepụtara.n'afọ 1960.PIPES nwere pKa n'akụkụ pH physiological nke na-eme ka ọ baa uru na ọrụ omenala cell.Edeela ya iji belata mfu egbugbere ọnụ mgbe ị na-ebufe glutaraldehyde histology n'ime anụ ahụ osisi na anụmanụ. Onye na-echekwa ihe nwere pKa nso pH physiological.
Antimicrobial peptide magainin 2 na-etolite pores n'ime membranes lipid ma na-ebute mwepu nke ihe dị n'ime cell.Ọ bụ ezie na ngafe a nwere ike ịbụ isi ihe na-akpata ọrụ nje bacteria, usoro e si emepụta pore ka aghọtachaghị nke ọma.Ya mere, anyị nyochara nke ọma mmekọrịta nke magainin 2 na akpụkpọ anụ lipid na-eji otu nnukwu unilamellar vesicles (GUVs).Njikọ nke magainin 2 na akpụkpọ anụ lipid nke GUVs mụbara mgbanwe nke akụkụ ahụ na mpaghara akpụkpọ ahụ, δ, nke kwekọrọ na ntinye elu nke magainin 2, X. Nke a na-egosi na ọnụ ọgụgụ na-agbanwe agbanwe nke magain 2-mere abụọ. -Mgbanwe steeti site na ọnọdụ adịghị emebi emebi gaa na pore steeti mụbara nke ukwuu na mmụba na δ.Esemokwu nke akpụkpọ ahụ lipid na-eso ọchịchọ nke GUV mekwara ka nhazi nke 2 na-akpata magainin.Iji kpughee ebe magainin 2 dị, mmekọrịta nke carboxyfluorescein (CF) - nke akpọrọ magainin 2 (CF-magain 2) nwere otu GUV nwere nyocha fluorescent nke mmiri na-agbaze, AF647, nyochara site na iji microscopy confocal.Na enweghị esemokwu n'ihi ọchịchọ, mgbe mkparịta ụka magainin 2 gasịrị, fluorescence ike nke GUV rim n'ihi CF-magainin 2 mụbara ngwa ngwa na uru na-adịgide adịgide, nke nọgidere na-adịgide adịgide ruo ogologo oge, na na 4-32 s tupu. mmalite nke ntapu nke AF647 ike rim malitere ịrị elu ngwa ngwa ruo uru kwụ ọtọ ọzọ.N'ụzọ dị iche, na ọnụnọ nke esemokwu ahụ, ọ dịghị mmụba na rim ike obere oge tupu mmalite nke ntanye ahụ.Nsonaazụ ndị a na-egosi na magainin 2 enweghị ike ịtụgharị site na mpụta gaa na monolayer n'ime ruo obere oge tupu e mepụta pore.Dabere na nsonaazụ ndị a, anyị kwubiri na pore magainin 2 na-akpata bụ pore na-eme ka ọ gbatịa ma gbatịa monolayer n'ime bụ isi ihe na-eme ka ọkpụkpụ ahụ guzosie ike.
