Ngwaahịa

A na-akọwa usoro immunoassay siri ike nke siri ike maka mkpebi nke progesterone na plasma mmadụ, nke na-eji chemiluminescence dị ka njedebe njedebe.Otu progesterone-isoluminol conjugate na-eje ozi dị ka akara chemiluminescent.Otu akụkụ nke IgG nke antiserum na progesterone-11 alpha-hemisuccinate bovine serum albumin ka a na-etinye na mgbidi nke tubes ule Lumacuvettes P polystyrene.Mgbe mmeghachi omume nke njikọ ahụ gasịrị, a na-asacha cuvettes.Mmụba ọkụ nke njikọ ejikọta na oxidation na sistemu H2O2-microperoxidase na pH 13 belatara site na ịbawanye ụba nke progesterone n'efu n'ụzọ log-linear karịa oke 15-1000 pg/tube.Chemiluminescence immunoassay bụ atụnyere radioimmunoassay n'ihe gbasara uche, nkọwapụta, nkenke na izi ezi.Nnwale ahụ na-enye uru nke ọsọ na mfe nke akpaaka.Na mgbakwunye, usoro a anaghị agụnye iji radioisotopes ma ọ bụ nzọụkwụ centrifugation.