Ngwaahịa

Na usoro kinetic angiotensin-converting enzyme (ACE), ihe nchekwa bara uru na nke kachasị mma bụ 80 mmol/L borate buffer na pH 8.2 (37 degrees C).A na-achọpụta usoro nkwụsịtụ na mmeghachi omume ahụ, a na-atụkwa aro ntinye nkeji 5 nke mkpụrụ na plasma tupu ntule kinetic.Mkpụrụ ahụ, N-[3- (2-furyl)acryloyl]-L-phenylalanylglycylglycine (FAPGG), a na-eme ka ntinye uche dị elu na 1.0 mmol/L na nha nha nha dị na 345 nm iji hụ na nha nha.Usoro a tụrụ aro na-eji oke 1:9 plasma-to-reagent ruru.Ogologo ahịrị ahịrị nke nyocha ahụ gbatịrị ihe ruru 170 U/L, na-anọchite anya hydrolysis nke mkpụrụ 25%.A na-ekpebi absorptivity FAPGG site na ịlele ọdịiche dị na nnabata n'etiti 1.0 mmol/L FAPGG na ngwọta ngwaahịa.A na-enyocha iguzosi ike n'ezi ihe nke ogologo ogologo site n'ịchọpụta uru nnabata a na-atụ anya ya nke ngwọta FAPGG, yana ngbanwe 1.0-nm site na 345 nm na-agbanwe nnabata ahụ site na 15.5%.Nkenke nke nyocha ACE na ihe dịka 60 na 100 U/L bụ 3.5% na 2.4% n'ime ogbe yana 2.9% na 2.6% n'etiti ogbe, n'otu n'otu.Oge ntụaka (2.5th ruo 97.5th percentiles) bụ 41-139 U/L, ọ nweghịkwa ọdịiche dị n'etiti ụkpụrụ maka ndị nwoke na ụmụ nwanyị.