Ngwaahịa

1.Metabolite usoro profiling bụ ngwaọrụ dị mkpa maka nha ọdọ mmiri metabolite na usoro ndu.Ọ bụ ezie na ọtụtụ ụzọ profaịlụ metabolite na-akọ oke ike ma ọ bụ dabere na ụkpụrụ dị n'ime ole na ole na-anọchite anya metabolites niile, ihe kacha mkpa maka nkọwa ọnụọgụ nke ọdọ mmiri metabolite na sel na mmiri mmiri bụ mkpebi siri ike.Anyị na-akọ ebe a usoro nkọwapụta metabolite ezubere iche maka chromatography gas chromatography/tandem mass spectrometry (GC/MS/MS) na-eme ka ọnụọgụgụ zuru oke nke metabolites niile achọpụtara.Usoro a gbadoro ụkwụ na mwepu methyl chloroformate na nhapụta site n'ịpịpụta n'omume nwere ụkpụrụ metabolite nke ewepụtara ya na reagents deuterated derivatization.The omenala eletrọn mmetụta ionization na-dochie na nti kemịkalụ ionization ebe nke ikpeazụ na a ukwuu ibu ruo n'ókè na-echebe molekul ion na ndị ọzọ elu molekụla arọ iberibe.Nke a mere ka ọ dị mfe ịhọrọ mgbanwe MS/MS pụrụ iche n'etiti ọtụtụ metabolites na-akpakọrịta.Ugbu a, usoro akwụkwọ ọgụgụ GC/MS/MS nwere metabolites 67 nkịtị bụ nke ọtụtụ n'ime ha so na otu amino na nonamino organic acid.Anyị na-egosi ngwa ngwa nke usoro na mmamịrị na serum samples.Usoro a bụ mmụba dị ịrịba ama nke usoro dị ugbu a maka profaịlụ metabolite nke GC/MS nke amino acid na nonamino organic acid.

2.24S-Hydroxycholesterol (24OHC) na 27-hydroxycholesterol (27OHC) bụ abụọ structurally yiri oxysterols nke dị iche iche si malite - nke mbụ fọrọ nke nta ka nanị kpụrụ na ụbụrụ na nke ikpeazụ kpụrụ ka nta n'ókè na ụbụrụ karịa na ọtụtụ ndị ọzọ akụkụ.IHE A GA-anwale: Mmebi Neuronal na / ma ọ bụ demyelination na-akpata mmụba nke 24OHC site na ụbụrụ n'ime mmiri cerebrospinal (CSF), ebe ntụpọ ọbara-ụbụrụ ụbụrụ na-akpata mmụba nke 27OHC site na mgbasa n'ime CSF.Isotope dilution-mass. A na-eji spectrometry mee ihe iji nyochaa oxysterols abụọ dị na CSF na plasma site na ihe karịrị ndị ọrịa 250 dị iche iche na-arịa ọrịa akwara na ọrịa geriatric. Ụdị CSF-ọkwa nke abụọ oxysterols na-emetụta ọrịa dị iche iche karịa ọkwa plasma.Ndị ọrịa nwere ọrịa demyelinating na-arụ ọrụ abawanyela ọkwa 24OHC na CSF nwere oke 24OHC/27OHC dị elu.Ndị ọrịa nwere maningitis n'ozuzu ha nwere ọkwa dị elu nke steroid abụọ nwere oke 24OHC/27OHC dị ala.Ndị ọrịa nwere ọrịa Alzheimer abawanyela ọkwa 24OHC na CSF yana mmụba dị ntakịrị na 27OHC.Ndị ọrịa nwere otutu sclerosis nwere ọchịchọ inwe ọkwa dị elu nke 24OHC n'oge oge na-arụ ọrụ na oke 24OHC / 27OHC. Ntụle nke oxysterols abụọ dị na CSF na plasma nwere ike ịgbakwunye nke ọma na usoro biochemical dị ugbu a maka nyocha nke ọrịa akwara.