Ngwaahịa

Monogalactopyranosides nke fluorescein na fluorescein methyl ester: njikọ, enzymatic hydrolysis site biotnylated β-galactosidase, na mkpebi siri ike nke ntụgharị ntụgharị.

Fluorescein monoglycosides (d-galactopyranoside (FMG) na d-glucopyranoside) na methyl ester (MFMG) ha akwadoro site na acetobromoglucose / galactose na fluorescein methyl ester na mkpụrụ dị mma.Emeela nnwale hydrolysis nke enzymatic (iji biotinylated β-galactosidase) nke ihe mgbapụta galacto wee gbakọọ paramita kinetic.Achọpụtala mmụba nke 15-20 ugboro nke fluorescence n'oge hydrolysis.Achọpụtala mmụba n'ahịrị nke fluorescence n'oge dị mkpirikpi na obere ntinye nke mkpụrụ, na-eme ka ogige ndị a bara uru na nyocha nyocha maka galactosidases.Ogo nke uru Michaelis-Menten na-adịgide (Km) maka MFMG dị elu karịa nke FMG na-atụ aro mgbanwe mgbanwe mgbanwe nke mkpụrụ fluorogenic.Uru km maka β-Gal biotinylated nwere FMG dị ala karịa nke ahụ maka enzyme nke ala.Nlebanya a na-egosi njikọ dị elu nke mkpụrụ ndụ biotinylated ma e jiri ya tụnyere enzyme nke ala.Atụlela ọnụọgụ mgbasa ozi ntụgharị, maka ma mkpụrụ nke fluorogenic na ma ngwaahịa ahụ, na-eji spectroscopy fluorescence correlation spectroscopy.Atụle ọnụọgụ mgbasa ozi ntụgharị maka ihe ndị na-emepụta fluorogenic na ngwaahịa enzymatic hydrolysis ka ha yitere, n'ogo nke 3.5-4.5 × 10-10 m2 s-1.Ya mere nkwalite ma ọ bụ nkwụghachi azụ nke enzymatic kinetics n'ihi ọdịiche dị na ntụgharị ntụgharị nke mkpụrụ na ngwaahịa anaghị apụta ìhè.