Ngwaahịa

A na-ahụkarị mgbasa nke protein n'ime oghere mmiri siri ike na-akwalite nguzobe nke mkpokọta soluble na nnukwu, nke a na-ahụ anya, na nke a na-ahụ anya, mana usoro isi na usoro a na-esikarị ike nyocha ozugbo.Mkpokọta agbaziri site na mgbasa ozi na mmiri-silicon oxide (SiOx) interfaces, dị ka ihe dị n'elu iko mmiri, bụ ọrụ nke pH na ike ionic maka alpha-chymotrypsinogen (aCgn) na maka mgbochi monoclonal (IgG1).Otu mkpụrụ ndụ na-erute ekwenyere neutron reflectivity maka akwa protein agbadoro iji sachaa elu SiOx, yana mgbe usoro “ichacha” gachara.Mkpokọta ndị enwetara na ngwọta ka ejiri nwayọọ "sachasị" elu ahụ bụ mgbasasa neutron, microscopy, na fluorescence spectroscopy.Molecules IgG1 gbadoro ụkwụ na isi "dị larịị" megide elu SiOx, yana akwa protein bụ isi agbazere ruo ntakịrị ntakịrị, ebe a na-ehichapụ ihe mkpuchi na-agbasa ngwa ngwa.Acgn ụmụ irighiri ihe na-eguzogide ọgwụ mgbe ha pụtara na a ga-ekpughere ha na interface ahụ, mana ewepụghị ya ngwa ngwa.Maka ikpe ebe njide siri ike mere, protein nke desorb bụ ngwakọta nke monomer na obere mkpokọta HMW (maka aCgn) ma ọ bụ ihe ndị a na-ahụ anya (maka IgG1).Mgbanwe na adsorption na/ma ọ bụ mkpughe na pH gosiri na mmekọrịta electrostatic dị mkpa n'ọnọdụ niile.