Ngwaahịa

A na-akọwa usoro nsị maka iji ya na akụkụ anụ ahụ glycol methacrylate agbakwunyere nke na-adịghị emetọ ihe ntinye plastik ma ọ bụ wepụ akụkụ ahụ na slides iko.Ihe agba agba bụ celestine acha anụnụ anụnụ B. A na-akwado ya site n'ịgwọ 1 g nke agba na 0,5 ml sulfuric acid.A na-agbaze ya na ngwọta na-esonụ.Tinye 14 ml glycerine na 100 ml 2.5% ferric ammonium sulfate ma kpoo ngwọta na 50 C. N'ikpeazụ dozie pH na 0.8 ruo 0.9 Ihe ngwọta nke acid acid mejupụtara 0.075% ponceau de xylidine na 0.025% acid fuchsin na 10% acetic acid.A na-emikpu ihe mmịfe nwere akụkụ plastik akpọnwụwo n'ime ngwọta celestine na-acha anụnụ anụnụ maka nkeji ise yana n'ime ngwọta ponceau-fuchsin maka nkeji iri na mmiri na-etinye aka.Mgbe ịsachara ikpeazụ, akụkụ ndị ahụ na-ekpo ọkụ ma kpuchie ya.Usoro nsị a na-agba agba anụ ahụ fọrọ nke nta ka ọ bụrụ otu usoro hematoxylin na eosin.