Ngwaahịa

Bicine bụ ihe nchekwa amino acid zwitterionic, na-arụ ọrụ na pH 7.6-9.0 (pKa nke 8.26 na 25°C).Ihe nchekwa akwadoro maka ọrụ biochemical dị ala.A na-eji Bicine maka nkwadebe nke mkpụrụ osisi kwụsiri ike maka mkpebi siri ike nke serum guanase.Ebipụtala ojiji nke bicine na usoro chromatography mgbanwe ion dị mkpa maka mkpebi protein.A na-eji bicine mee ihe na peptide na protein crystallization.Ọmụmụ ihe ọmụmụ nke quaternary-state analogue complex nke creatine kinase ejiri bicine na nchekwa mmeghachi omume.A kọwapụtala sistemu nchekwa multiphasic maka SDS-PAGE nke protein na peptides na-etinye bicine.

Otu mgbidi alveolar na-edobere ọmụmụ ihe ogologo ogologo oge na saline na Bicine (0.2 M) na-enwe ọganihu na-aga n'ihu na esemokwu anụ ahụ (TTD).Anyị enyochala mmetụta nke ngwọta dị iche iche na TTD a wee chọpụta mgbanwe ndị kwekọrọ na ultrastructure.A na-ekesa parenchyma akpa ume na otu mgbidi alveolar (30 X 30 X 150 microns) na saline phosphate-buffered (0.15 M).N'ịbụ ndị a na-ebufe na bath bath nke ogologo oge, a na-emikpu anụ ahụ na Bicine, saline, ngwọta Hank siri ike, 0.25% Alcian blue in saline, ma ọ bụ sodium dodecyl sulfate solution, maka oge mgbanwe.N'ịbụ nke gbagharịrị site na ndọtị enyere ya na ike kacha elu tụrụ ka oge na-aga, etinyere otu anụ ahụ na glutaraldehyde/tannic acid buffered wee hazie ya maka microscopy eletrọn.Otu mgbidi alveolar na-emikpu na saline ma ọ bụ Bicine gosipụtara TTD na-aga n'ihu.Vacuoles ma ọ bụ oghere pụtara na interstitium bụ nke na-enwe mgbagwoju anya cell na-aga n'ihu na TTD.A hụrụ n'ime 0.3 h, mgbanwe ndị ahụ dị elu na 0.6 h.Na sodium dodecyl sulfate (70 mM), Otú ọ dị, ọ dịghị TTD na structurally ọ dịghị interstitium, na naanị okpuru ulo membran na protein fibrous fọdụrụ.Na ngwọta Hank siri ike ma ọ bụ 0.25% Alcian blue a na-echekwa matrix interstitial, morphology cell na esemokwu anụ ahụ nke ọma maka awa 1.Ọmụmụ ihe a na-egosi na nkwụsị nke matriks interstitial na-apụta na saline ma ọ bụ Bicine, matriks na-adịghị mma dị mkpa maka ichekwa esemokwu anụ ahụ.