Ngwaahịa

1.The modulation nke ọrụ nke enzymes metụtara carbohydrate metabolism dị mkpa maka nduku oyi-induced sweetening (CIS).A na-eme ka mkpụrụ ndụ mkpịsị aka RING ọhụrụ SbRFP1 wee hụ na okwu ya na-adị oyi na tubers nduku nke genotypes na-eguzogide CIS.Mgbanwe nke SbRFP1 na poteto gosipụtara ọrụ ya na igbochi β-amylase na ọrụ invertase, nke mere ka ọ daalata starch na mbibi sucrose na mkpokọta nke ibelata shuga na tubers oyi echekwabara.Nchọpụta ndị a siri ike na-egosi na SbRFP1 nwere ike ịrụ ọrụ dị ka onye na-achịkwa BAM1 na StvacINV1 na-adịghị mma iji mebie mkpokọta nke ibelata sugars na usoro nduku CIS.

2.Ebumnuche nke ọrụ a bụ iji mụọ mmetụta nke uzuoku nri na pectin methylesterase (PME) na endogenous α- na β-amylase omume na dị iche iche anụ ahụ (cortex na pith) nke raw na okpomọkụ-emeso poteto cv.Agria.Ahọpụtara okpomọkụ isi nri atọ dị iche iche (55, 70 na 85 Celsius C).Maka ule nri ọ bụla, a na-edekọ profaịlụ oge okpomọkụ ma gosipụta ogo nke nri n'ihe gbasara nri nri. Isi nri Steam nyere aka mee ka PME rụọ ọrụ nke ọma na 55 Celsius C na iji belata ọrụ ya na nhazi nhazi ikpeazụ (85 ° C). , na ọnụ ọgụgụ kachasị elu na cortex (0.3745 ± 0.0007 µmol galacturonic acid (GA) g (-1) ịdị arọ ọhụrụ (FW) min (-1) ) ma e jiri ya tụnyere pith (0.2617 ± 0.0012 µmol GA g (-1) FW min (-1) ).A na-ewerekwa ọnụnọ nke okpomọkụ-labile na ikpo ọkụ na-ekpo ọkụ nke PME na anụ ahụ poteto a na-atụle.Ọgwụgwọ okpomọkụ site na uzuoku butere mbelata dị ukwuu na mmemme endogenous α- na β-amylase n'anụ ahụ abụọ ma e jiri ya tụnyere raw pota, n'agbanyeghị na enweghị nkwụsị zuru oke.Starch-eweda enzymes na-hụrụ na-dị iche iche na-ekesa na raw tuber.Steam nri emetụta n'ụzọ dị iche iche na-enyocha ihe fọdụrụ enzymatic ọrụ na-atụle anụ ahụ nduku cv.Agria.Achọrọ nyocha ọzọ iji kwado nsonaazụ enwetara.