alfa-Amylase CAS: 9000-90-2 C10H13FN2O4
| Nọmba katalọgụ | XD90389 |
| Aha ngwaahịa | Alfa-Amylase |
| CAS | 9000-90-2 |
| Usoro ihe omimi | C10H13FN2O4 |
| Ibu molekụla | 244.22 |
| Koodu tarifu akwadoro | 35079090 |
Nkọwapụta ngwaahịa
| Ebe mgbaze | 66-73°C |
| Ọdịdị | Ọcha ntụ ntụ |
Otu usoro ọgwụgwọ dị mkpa maka ọgwụgwọ ụdị ọrịa shuga mellitus nke 2 bụ iji ọgwụ ndị nwere ike ibelata hyperglycemia postprandial site na igbochi enzymes mgbaze carbohydrate.Ọmụmụ ihe dị ugbu a nyochara nsonaazụ bioassay na-eduzi mmịpụta na akụkụ nke mkpụrụ osisi a mịrị amị pericarp nke Phaleria macrocarpa, ahịhịa ọdịnala na-egbochi ọrịa shuga, na α-glucosidase na α-amylase, iji ghọta usoro mgbochi ọrịa shuga ha, dịka. nakwa dị ka ha ga-ekwe omume attenuation edinam on postprandial glucose mmụba.Methanol wepụ (ME), nwetara site sochiri ihe mgbaze mmịpụta, ya kasị dị irè mmiri mmiri-mmiri n-butanol nta (NBF) na flash kọlụm chromatographic sub-iribiri (SFI), e atụle. maka in vitro α-glucosidase (yist) na α-amylase (porcine) mgbochi ọrụ.Ọzọkwa, a na-eme nyocha nke vivo na streptozotocin na-ebute ọrịa shuga (SDRs) site na iji ọnụ glucose ọnụ, sucrose na starch tolerance tests. N'ebe kachasị elu nke a na-arụ ọrụ (1 00 μg / ml), NBF gosipụtara mgbochi kachasị elu megide α-glucosidase. 75%) na α-amylase (87%) na vitro (IC50 = 2.40 ± 0.23 μg / ml na 58.50 ± 0.13 μg / ml, n'otu n'otu) n'ụdị nke dabere na dose;mmetụta achọpụtara dị ka 20% dị elu karịa acarbose (55%), ọkọlọtọ α-glucosidase inhibitor (IC50 = 3.45 ± 0.19 μg / ml).ME na SFI gbochiri α-glucosidase (IC50 = 7.50 ± 0.15 μg/ml na 11.45 ± 0.28 μg/ml) na α-amylase (IC50 = 43.90 ± 0.19 μg/ml na 69.82 ± μg / ml na 69.80 μg). nke dị obere.N'ime ọmụmụ vivo na oke ọrịa shuga, NBF na SFI belatara ọkwa glucose ọbara kacha elu (PBG) nke ọma site na 15.08% na 6.46%, yana mpaghara n'okpuru usoro ndidi (AUC) site na 14.23% na 12.46%, n'otu n'otu, mgbe ihe ịma aka sucrose nke ọnụ gasịrị. (P <0.05);si otú a na-akwado ihe a hụrụ na vitro edinam.E gosipụtakwara mmetụta mbelata ndị a na PBG na AUC na glucose na ule nnabata starch, mana n'ókè dị nta. Nchọpụta ndị a na-ekpughe na P. macrocarpa nwere ike belata hyperglycemia na ma in vitro na ọnọdụ vivo site n'ike na-egbochi carbohydrate hydrolysing enzymes, na-eme ya. ihe ọkụkụ dị mma maka ị nweta ogige eke maka njikwa ụdị ọrịa shuga mellitus nke 2.
