Ngwaahịa

Protein phosphorylation na-achịkwa ọrụ cellular dị iche iche ma na-ekere òkè dị mkpa na mmalite mmepe nke osisi.Iji mejuo ma gbasaa na nyocha gara aga nke protein phosphorylation na Arabidopsis seedlings, anyị jiri ụzọ ọzọ na-ejikọta mmịpụta protein n'okpuru ọnọdụ ndị na-abụghị denaturing na immobilized metal-ion affinity chromatography (IMAC) nke phosphoproteins na-emebibeghị na mpempe akwụkwọ Rubisco-depleted, sochiri ya na njirimara. na-eji gel electrophoresis abụọ (2-DE) na mmiri chromatography-tandem mass spectrometry (LC-MS/MS).In-gel trypsin mgbaze na nyocha nke ntụpọ gel ahọpụtara chọpụtara 144 phosphorylated peptides na residues, nke naanị 18 phosphopeptides na 8 phosphosites ka achọtara na PhosPhAt 4.0 na P3DB Arabidopsis thaliana phosphorylation saịtị ọdụ data.Ihe karịrị ọkara nke 82 ndị a chọpụtara phosphoproteins nọ na-etinye aka na carbohydrate metabolism, photosynthesis/respiration ma ọ bụ mechani SMS nzaghachi nrụgide oxidative.Ịba ụba nke phosphoproteins na-emebibeghị tupu 2-DE na LC-MS/MS na-egosi na ọ na-eme ka nchọpụta nke phosphorylated threonine na tyrosine residues tụnyere ụzọ ndị na-eji nkwalite ọkwa peptide dị elu, na-atụ aro na ụzọ abụọ a dịtụ aka n'ihe gbasara saịtị phosphorylation.Ntụle nsonaazụ maka mkpụrụ osisi na-eto eto na nke enwetara na mbụ maka akwụkwọ Arabidopsis tozuru oke achọpụtara protein ise dị iche iche nke phosphorylated dị na anụ ahụ ndị a, na-egosipụta ikike nke usoro a maka inyocha ike nke protein phosphorylation n'oge mmepe osisi.