Ngwaahịa

Emepụtara aptasensor dị mma nke ejiri graphene oxide (GO) mee ihe dị ka ihe ngosi maka ma electrochemical impedance spectroscopy na electrochemiluminescence (ECL).Emepụtara aptasensor site n'ịchịkọta nyocha nke ECL nke thiolated adenosine triphosphate binding aptamer (ABA) nke ejiri Ru complex (Ru(bpy)3(2+) mmepụta) n'elu igwe nanoparticle ọla edo (AuNP) gbanwere carbon glassy. electrode (GCE).ABA immobilized na AuNP gbanwetụrụ GCE nwere ike ịgbasa GO n'ụzọ siri ike n'ihi mmekọrịta siri ike n'etiti ABA na graphene oxide;ECL quenching nke Ru complex na-ewe ebe n'ihi ike nyefe na eletrọn nyefe, na a nnukwu mmụba nke electron nyefe nguzogide (Ret) nke electrode.Mgbe ọ nọ n'ihu adenosine triphosphate (ATP), ABA na-ahọrọ ịmepụta ogige bioaffinity ABA-ATP, nke na-adịghị ike na graphene oxide ma mee ka graphene oxide pụọ na elu electrode, si otú a na-enye ohere nkwalite mgbaàmà ECL, na na njikọ na mbelata nke Ret.N'ihi ịdị elu ECL quenching arụmọrụ, usoro pụrụ iche, na akụrụngwa eletrọnịkị nke graphene oxide, ike Ret na ECL megide logarithm nke itinye uche ATP bụ ahịrị n'obosara site na 10 pM ruo 10 nM nwere oke nchọpụta dị ala nke 6.7. pM ruo 4.8 pM, n'otu n'otu.Aptasensor a tụrụ aro gosipụtara ngbanwe dị mma, nkwụsi ike na nhọrọ pụtara ìhè, yana enwere ike ịmata ATP nke ọma na analogues ya.Nke ka nke ọma, atụmatụ ECL aptasensor nke ọma a dabere na GO na-eme ma dị ka ihe nrịbama elektrọnịkị na akara ECL bụ n'ozuzu yana enwere ike ịgbatị ngwa ngwa na mmemme njikọ ndu ndị ọzọ.