Ngwaahịa

1.Optically arụ ọrụ D-2-hydroxybutanoate bụ ihe dị mkpa ụlọ ngọngọ etiti maka ọgwụ na biodegradable poly (2-hydroxybutanoate).Mkpebi kinetic nke agbụrụ 2-hydroxybutanoate nwere ike ịbụ akwụkwọ ndụ akwụkwọ ndụ ọzọ na-achọsi ike maka mmepụta D-2-hydroxybutanoate.N'ime ọrụ a, D-2-hydroxybutanoate na ntinye uche dị elu (0.197 M) na oke enantiomeric (99.1%) bụ nke NAD na-adabere na L-lactate dehydrogenase (L-iLDH) nwere biocatalyst.2-Oxobutanoate, ihe ọzọ dị mkpa dị n'etiti, na-ejikọta ya na nnukwu mkpokọta (0.193 M).N'iji usoro mgbanwe ion dị mfe na macroporous anion exchange resin D301, D-2-hydroxybutanoate kewapụrụ na usoro biotransformation na mgbake dị elu nke 84.7%.

2.E gosipụtara njikọ asymmetric nke amino acid na-ekwekọghị n'okike site na omega-transaminase sitere na Vibrio fluvialis JS17.Ejiri L-2-Aminobutyric acid sitere na 2-oxobutyric acid na benzylamine nwere oke enantiomeric dị elu karịa 99%.Mmeghachi omume gosipụtara mmechi ngwaahịa siri ike site na benzaldehyde, nke meriri site n'iji usoro mmeghachi omume biphasic wepụ ngwaahịa mgbochi ahụ na mpaghara mmiri.Na mmeghachi omume biphasic na-ahụkarị (50 mM 2-oxobutyric acid, 70 mM benzylamine na 2.64 U / ml enzyme dị ọcha) na-eji hexane dị ka onye na-emepụta ihe, ntụgharị nke 2-oxobutyric acid ruru 96% na 5 h ebe naanị 39% ntughari nwetara na-enweghị. mmịpụta ngwaahịa.