Ngwaahịa

Cysteine ​​​​(Cys) na-arụ ọrụ dị mkpa n'ọtụtụ usoro physiological nke mkpụrụ ndụ eukaryotic na nchọpụta ya na sel bụ ihe dị mkpa.Agbanyeghị, glutathione (GSH), homocysteine ​​​​, N-acetyl-L-cysteine ​​​​na thiols ndị ọzọ na-egbochi nchoputa nke Cys.Karịsịa, GSH na-etinye aka na nchọpụta nke cellular Cys (30-200 μM) n'ihi nnukwu ntinye uche intracellular ya (1-10 mM).N'ime ọrụ a, a na-ewepụta ihe nyocha na-apụ apụ na fluorescent (HOTA) maka nchọpụta Cys.Nyocha a nwere ma ezi uche dị mma na nhọrọ na-eju afọ maka nchọpụta cellular Cys: na mgbakwunye nke 200 μM Cys, ike ọkụ nke nyocha (10 μM) kwalitere 117-fold na njedebe nchọpụta gbakọrọ ka ọ bụrụ 13.47 μM, nke dị ala. karịa mkpokọta Cys cellular;Nchọpụta ahụ chọpụtakwara nke ọma 30-200 μM cysteine ​​n'elu 1-10 mM glutathione.Ya mere, ejiri nyocha HOTA mee nnwale onyonyo cell.Ọzọkwa, nsonaazụ nke thiol-blocki ng na njikọ GSH na-egbochi nnwale gosipụtara na ikuku intracellular sitere na mmekọrịta dị n'etiti Cys na HOTA.