Ngwaahịa

Njirimara: 3,3',5,5'-Tetramethylbenzidine bụ ọcha crystalline ntụ ntụ, odorless, enweghị ụtọ, insoluble na mmiri, mfe soluble na acetone, ether, dimethyl sulfoxide, dimethylformamide, wdg Organic solvents.

Nkwadebe: 3,3,5,5-Tetramethylbenzidine bụ ihe dị mkpa reagent chromogen.Iji 2,6-dimethylaniline dị ka akụrụngwa, site na ịgbalite, njikọta oxidative na ịdị ọcha, a na-enweta 3,3,5,5-tetramethylbenzidine dị ọcha, na mkpokọta mkpụrụ ruru 65%.

Ọrụ ndu: TMB (BMblue) bụ mkpụrụ chromogenic maka immunohistochemistry na ELISA.

Na-eji: Ngwaahịa a bụ ihe na-abụghị carcinogenic nọchiri anya benzidine 1 (Ames test negative), nke dabara dị ka mkpụrụ nke peroxidase maka nyocha immunosorbent jikọtara enzyme.Mkpụrụ ahụ na-ewepụta ngwaahịa ikpeazụ na-acha anụnụ anụnụ nke nwere ike ịgụ spectrophotometrically na 370 ma ọ bụ 620-650 nm.Enwere ike ịkwụsị mmeghachi omume TMB na 2MH2SO4 (tụgharịrị edo edo) wee gụọ na 50 nm na 4Chemicalbook.Reagent nke nwere mmetụta dị nro na nke akọwapụtara maka ịchọpụta ọbara, ịlele haemoglobin, na ịtụ peroxidase.

Na-eji: Ọhụrụ na nchekwa chromogen reagent;TMB ejirila nwayọọ nwayọọ dochie carcinogen benzidine siri ike na ihe ndị ọzọ na-emepụta carcinogenic benzidine, a na-ejikwa ya na nyocha ụlọ ọgwụ, nyocha nyocha, nchọpụta mpụ na nlekota gburugburu ebe obibi na mpaghara ndị ọzọ;karịsịa na Nnwale biochemical nke ụlọ ọgwụ, TMB, dị ka ihe ọhụrụ maka peroxidase, ejirila ya mee ihe na enzyme immunoassay (EIA) na enzyme-linked immunosorbent assay (ELISA);a na-ejikarị ya eme ihe n'ọrụ ndị a: nchọpụta mkpịsị aka ọbara mgbaasị;Nchọpụta ngwa ngwa nke mmanya na-egbu egbu;nkwadebe nke mpempe ule mmamịrị;nchọpụta nje ịba ọcha n'anya;ule nchọpụta ime ime;mkpebi siri ike nke glucose, hemoglobin, albumin n'ime ọbara na mmamịrị;nyocha ọbara mgbaasị fecal;Mkpebi nke uru granulocyte na ọbara, steroid, nchọpụta nke homonụ mmekọahụ;mkpebi nke ọrụ enzymatic;nchọpụta nke antigens, ọgwụ nje na mkpụrụ ndụ ihe nketa