Ngwaahịa

Biosensor microRNA (miRNA) na-enwe mmetụta nke ukwuu nke na-eji ruthenium oxide nanoparticle (RuO(2) NP) –bido polymerization nke 3,3'-dimethoxybenzidine (DB) na ntinye ihe atụ nke miRNA nke poly insulating (3,3'-dimethoxybenzidine) A kọwara ihe nkiri (PDB) n'ọrụ a.Emebere biosensor site na monolayer agwakọta nke oligonucleotide capture probes (CPs) na 4-mercaptoaniline na electrode ọla edo.Na-esochi ngwakọ ya na ruO(2) NP nwere akara miRNA, ngwakọta nke DB/H(2)O(2) na pH 5.0 0.10 M acetate buffer ka etinyere na biosensor.Ndị RuO (2) NP na-eje ozi dị ka polymerization initiator / ihe mkpali maka polymerization nke DB.Na strands anionic miRNA ngwakọ na CP n'efu na-eje ozi dị ka ndebiri, na-eduzi ntinye nke PDB.Ọnụ ego PDB echekwara na ike mkpuchi ya jikọtara ya na ntinye nke miRNA ebumnuche na ngwọta.Nnwale nyocha spectroscopic electrochemical impedance egosila na mmekọrita nkwụghachi ụgwọ-nfefe-ntụgharị uche sitere na 6 .0 fM ruo 2.0 pM nwetara mgbe 60 min nke nnabata na ngwakọta DB/H(2)O(2).Enweghị njikọ njikọta n'etiti pre-miRNA na miRNA tozuru oke yana obere njikọ njikọ n'etiti ndị ezinaụlọ miRNA nwere njikọ chiri anya ọbụlagodi n'ọkwa na-enweghị otu ntọala.Biosensor nke dabere na impedance na-enye ụzọ mara mma maka ịkọwapụta okwu miRNA ma nwee ike mee ka mmepe nke sistemu profaịlụ miRNA multiplexing.