Ngwaahịa

Ihe dị iche iche nke fibroblasts orbital n'ime adipocytes tozuru okè na nchịkọta anụ ahụ nke adipose na-esote egosiwo na ọganihu nke Graves' orbitopathy (GO).Autophagy na-etinye aka na adipogenesis, mana amachaghị banyere ọrụ nke autophagy na mmalite na ọganihu nke GO.Ebumnuche nke ọmụmụ a bụ iji nyochaa ọrụ nke autophagy na pathogenesis nke GO. Orbital adipose / connective tissue explants sitere na ndị ọrịa nwere GO na site na ihe ndị nkịtị, yana ndị dịpụrụ adịpụ orbital fibroblasts.A kpaliri Adipogenesis site na iji ihe dị iche iche nwere ma ọ bụ na-enweghị hydrogen peroxide, a na-ejikwa autophagy mee ihe site na iji bafilomycin A1 na Atg5-ezubere iche ntutu isi mkpụmkpụ RNA (shRNA).Achọpụtara autophagosomes site na microscopy eletrọn.A tụlere nkwupụta nke mkpụrụ ndụ ihe nketa metụtara autophagy na ihe odide ederede metụtara adipogenesis site na ntule oge ntụgharị ntụgharị-polymerase na / ma ọ bụ nyocha Western blot.A na-enyocha mmụkọ mmiri ara ehi site na mmanụ Red O staining. Autophagic vacuoles dị ukwuu na sel GO karịa na sel ndị na-abụghị GO (p<0.05).Ngosipụta nke mkpụrụ ndụ ihe nketa metụtara autophagy dị elu nke ukwuu na anụ ahụ na sel GO karịa na ndị ibe ha na-abụghị GO, n'otu n'otu.Interleukin-1β mụbara LC3-II, p62, na protein Atg7 na sel GO.E gosipụtara mkpokọta autophagosome na ụbọchị 4 nke adipogenesis ma belata n'ụbọchị 10, yana nhazi nke lipid droplet.Ngosipụta nke protein LC3 na p62 mụbara n'ime awa 48 nke ọdịiche dị iche iche ma jiri nwayọọ nwayọọ na-ebelata site na ụbọchị 4 ruo 10. Ọgwụgwọ Bafilomycin A1 na Atg5 knockdown site na shRNA na-egbochi nchịkọta lipid droplet na okwu nkwụsị nke ihe nrịbama adipogenic. Autophagy na-abawanye na anụ ahụ GO na mkpụrụ ndụ ma e jiri ya tụnyere ya. anụ ahụ na-abụghị GO na mkpụrụ ndụ, na-atụ aro na autophagy na-ekere òkè na GO pathogenesis.Mgbanwe nke autophagy nwere ike ịbụ ebumnuche ọgwụgwọ maka GO.