Ngwaahịa

ATP-gated P2X3 nnabata bụ mkpa transducers nke nociceptive stimuli na ọ fọrọ nke nta ka ọ na-egosipụta nanị site sensory ganglion neurons.Na mouse trigeminal ganglion (TG), ọrụ nnabata P2X3 na-atụghị anya ya site na ọgwụ mgbochi nke natriuretic peptide receptor-A (NPR-A), na-akọwapụta ọrụ inhibitory nke peptides endogenous natriuretic peptides na nociception nke P2X3 na-anabata ya.Enweghị mgbanwe na okwu protein P2X3 na-egosi mgbanwe mgbagwoju anya nke usoro ya maka iweda ọrụ nnata P2X3 ka edoghị anya. Iji dokwuo anya usoro a na omenala TG òké, anyị kwụsịrị NPR-A na-egosi na siRNA nke endogenous agonist BNP, ma ọ bụ NPR-A. onye na-egbochi anantin.Ya mere, anyị nyochara mgbanwe na nkesa P2X3 na-anata n'ime oghere akpụkpọ anụ lipid raft, ọnọdụ phosphorylation ha, yana ọrụ ha na-ejikọta ya na patch clamping.Egbutela mmalite nke enweghị mmetụta P2X3 bụ otu usoro maka nkwalite anantin na-ebute ọrụ P2X3.Anant n'ime ngwa butere nnabata P2X3 ga-amasị ya na ngalaba raft lipid ma belata P2X3 serine phosphorylation, ihe omume abụọ na-adabereghị.Onye na-egbochi cGMP na-adabere na protein kinase na siRNA-mediated knockdown nke BNP ṅomiri mmetụta nke anantin.Anyị gosipụtara na na mouse trigeminal neurons endogenous BNP na-arụ ọrụ na ndị na-anabata NPR-A iji chọpụta ịda mbà n'obi nke ọrụ P2X3.Mgbochi Tonic nke ọrụ nnabata P2X3 site na ụzọ BNP/NPR-A/PKG na-eme site na usoro abụọ dị iche iche: P2X3 serine phosphorylation na redistribution receptor na-abụghị raft membrane compartments.Usoro ọhụụ nke njikwa nnabata nwere ike bụrụ ebumnuche maka ọmụmụ ihe n'ọdịnihu na-achọ ibelata ọrụ nnabata P2X3 dysregulated na mgbu na-adịghị ala ala.