ONPG CAS: 369-07-3 98.0% Nkeji na-acha ọcha ka gbanyụọ - White ntụ ntụ
| Nọmba katalọgụ | XD90006 |
| CAS | 369-07-3 |
| Aha ngwaahịa | ONPG(2-Nitrophenyl-beta-D-galactpyranoside) |
| Usoro ihe omimi | C12H15NO8 |
| Ibu molekụla | 301.25 |
| Nkọwa nchekwa | 2 ruo 8 Celsius C |
| Koodu tarifu akwadoro | 29400000 |
Nkọwapụta ngwaahịa
| Ịdị ọcha (HPLC) | Min.98.0% |
| Ọdịdị | Na-acha ọcha na-acha ọcha ntụ ntụ |
| Ngwọta(1% na mmiri) | Ngwọta doro anya, enweghị isi ma ọ bụ odo odo |
| Ọdịnaya Mmiri(Karl Fisher) | Oke.0.5% |
| Ntụgharị anya pụrụ iche [α]D20(c=1, H2O) | -65.0C ruo -73.0C |
Mkparịta ụka na ule ONPG (nnwale β-galactosidase)
A na-ajụkarị ajụjụ n'oge na-adịbeghị anya: 1. Gịnị kpatara enwere ike iji ule ONPG mee ka ọdịiche dị n'egbughị oge gbaa ụka lactose?2. Gịnị kpatara ọkọlọtọ mba ahụ ji kwuo na ọ dị mkpa iji 3% sodium chloride trisaccharide iron (ma ọ bụ trisaccharide iron) maka ule ONPG?3. Maka Vibrio parahaemolyticus, mgbe ị na-eme ule OPNG, gịnị kpatara a ga-eji tinye toluene dropwise dịka ọkọlọtọ?Gịnị bụ ọrụ?
Ụlọ ọrụ anyị enyochala ọtụtụ ozi, chịkọta ya, ma kesaa gị ya n'okpuru:
Ụkpụrụ: Aha Chinese nke ONPG bụ o-nitrobenzene-β-D-galactopyranoside.Enwere ike itinye ONPG n'ime galactose na odo o-nitrophenol (ONP) site na β-galactosidase, yabụ enwere ike ịchọpụta ọrụ β-galactosidase site na mgbanwe agba nke usoro omenala.
Lactose bụ shuga nke ọtụtụ microorganisms kwesịrị ịchọpụta.Metabolism ya chọrọ enzymes abụọ, otu bụ cell permease, lactose na-abanye n'ime sel n'okpuru ọrụ nke permease;nke ọzọ bụ β-galactosidase, nke na-eme ka lactose banye galactose.Lactose na glucose.β-Galactosidase nwekwara ike na-eme ihe ozugbo na ONPG ka hydrolyze ya n'ime galactose na odo o-nitrophenol (ONP).Enwere ike ime ya na awa 24, ọbụlagodi na fermenters na-egbu oge lactose.Ya mere, ọ na-akọwa nsonaazụ nlele nke ịhọrọ omenala 1 site na agar slant na inoculating ya na okirikiri zuru oke na 36 Celsius C maka 1-3h na 24h.Ọ bụrụ na emepụtara β-galactosidase, ọ ga-atụgharị edo edo na 1-3h, ọ bụrụ na enweghị enzyme dị otú ahụ, ọ gaghị agbanwe agba na 24h.
Dabere na enzymes abụọ a dị n'elu, enwere ike kewaa microorganisms n'ụdị ndị a:
1 lactose-fermenting (awa 18-24) nje bacteria nwere permease na β-galactosidase P + G +;
2 Ndị na-eme nri lactose na-egbu oge (na-ewe ihe karịrị awa 24) enweghị permease mana nwere galactosidase: P-G+.
3 fermenters na-abụghị lactose enweghị ma permease na galactosidase: P-G-.
Enwere ike iji ule ONPG mee ka ọdịiche dị n'etiti nje bacteria lactose-lag-fermenting (P-G+) na nje bacteria na-adịghị fermenting (PG-), dị ka:
1 Iche iche nke lactose fermenters (P-G+) na ndị na-abụghị lactose fermenters (P-G-).
(a) Citrobacter (+) na Salmonella arizonae (+) sitere na Salmonella (-).
(b) Escherichia coli (+) sitere na Shigella sonnei (-).
Kedu ihe kpatara eji eme nyocha ONPG site na iji omenala abalị na 3% sodium chloride ferric trisaccharide (iron trisaccharide)?Ụlọ ọrụ anyị enyochala ọtụtụ ozi, mana ọ nweghị nkwupụta doro anya.Naanị na ebe nrụọrụ weebụ FDA, e dere na "Inoculate omenala a ga-anwale n'elu atọ sugar iron agar slants na incubate maka 18 h na 37 ° C (ma ọ bụ ndị ọzọ kwesịrị ekwesị okpomọkụ, ma ọ bụrụ na achọrọ) . Nri (ma ọ bụ ndị ọzọ) agar slants nwere 1.0 % lactose nwekwara ike iji."pụtara: A na-agbanye nje bacteria na-anwale na igwe trisaccharide iron ma mebe ya na 37 ° C maka awa 18.Nri agar slants (ma ọ bụ ndị ọzọ) ọkara nwere 1% lactose dịkwa mma.Ya mere, a na-eche na trisaccharide iron media nwere lactose.Mgbe otu abalị gachara, nje bacteria emepụtala β-galactosidase na-arụ ọrụ nke ọma.N'iji nje bacteria ndị dị otú ahụ, ONPG nwere ike imebi site na β-galactosidase ngwa ngwa.Ihe ngosi nnwale na-adị ngwa ngwa ma gosipụta nke ọma.Na mgbakwunye, mgbakwunye nke toluene na mmiri ịsa mmiri maka nkeji 5 bụ ihe niile iji wepụta β-galactosidase nke ọma.
