NSP-SA-NHS CAS: 199293-83-9 odo crystaline ntụ ntụ
Nọmba katalọgụ | XD90129 |
Aha ngwaahịa | NSP-SA-NHS |
CAS | 199293-83-9 |
Usoro ihe omimi | C32H31N3O10S2 |
Ibu molekụla | 681.733 |
Nkọwa nchekwa | 2 ruo 8 Celsius C |
Nkọwapụta ngwaahịa
Ọdịdị | Odo kristal ntụ ntụ |
Nyocha | 99% |
Acridine ester (NSP-SA-NHS) Cas199293-83-9 na ogige ndị metụtara ya bụ aha njirimara chemiluminescent nke nkwụsi ike, ọrụ na uche karịrị ụfọdụ radioisotopes.Acridine esters nwere ike imeghachi omume na protein nwere otu amino isi.N'okpuru ọnọdụ ndị bụ isi, a na-edochi NHS dị ka ndị na-ahapụ, protein ahụ na-etolitekwa njikọ amide kwụsiri ike na acridine ester.Mgbe emechara mmeghachi omume ahụ, a na-ewepụ nnu acridinium ngafe site na kọlụm na-emebi emebi.
Ndị na-edozi akara Acridine anaghị achọ enzymatic catalysis iji na-ebunye ọkụ n'ihu alkaline hydrogen peroxide.Ụkpụrụ a kapịrị ọnụ na-enye ìhè bụ na na ngwọta alkaline hydrogen peroxide, acridine ester na-awakpo ions hydrogen peroxide iji mepụta dioxyethane na-adịghị eguzosi ike n'ihe na esemokwu, nke na-esiwanye ike na CO2 na acridone na-akpali akpali.Mgbe acridone laghachiri na steeti ala, ọ na-ewepụta photons nwere ogologo ogologo mmịpụta kacha nke 430 nm.Usoro luminescence a dị mkpụmkpụ (usoro a dum na-ewe ihe na-erughị 2 sekọnd), na atụmatụ na-akpali akpali aghaghị ịgbakwunye fotometer n'ime na onye nchọpụta foton;na mgbakwunye, ngwaahịa a nwekwara ike iji onye na-agụ microplate na-arụ ọrụ dị iche iche nke nwere autosampler maka nchịkọta data luminescence.Protein, peptides, antibodies, na nucleic acid niile nwere ike iji ngwaahịa a kpọọ aha.Acridine esters na-ebunye ọkụ ngwa ngwa n'okpuru mkpali nke alkaline hydrogen peroxide, yabụ enwere ike ịchọpụta ogige ndị akpọrọ site na ịnakọta foton.
A na-ejikarị ngwaahịa a maka: chemiluminescence na immunoassay, nyocha nnabata, nucleic acid na nchọpụta peptide na nyocha ndị ọzọ.