Ngwaahịa

Carbonic anhydrase activators: ọmụmụ mmalite izizi nke mmadụ nzuzo isoform VI nwere amino acid na amines.

Ihe nzuzo nke isozyme nke carbonic anhydrase nke mmadụ (hCA, EC 4.2.1.1), hCA VI, ka ejirila cloned, kwupụta ma mee ka ọ dị ọcha na usoro okwu nje.Usoro kinetic maka CO (2) mmeghachi omume hydration gosipụtara hCA VI nwere ak (cat) nke 3.4 x 10 (5) s (-1) na k (cat)/K (M) nke 4.9 x 10(7)M. (-1) s (-1) (na pH 7.5 na 20 degrees C).hCA VI nwere ọrụ catalytic dị ịrịba ama maka mmeghachi omume physiological, nke otu usoro nke ịdị ukwuu dị ka isoform CA I ma ọ bụ transmembrane, tumor-sociated isozyme CA IX.E gosipụtara usoro amino acids na amines ka ọ na-arụ ọrụ dị ka ndị na-arụ ọrụ CA VI, na-arụ ọrụ dị iche iche.l-His, l-Trp, na dopamine gosipụtara mmetụta na-arụ ọrụ CA VI na-adịghị ike (K (A) na nso 21-42 microM), ebe d-His, d-Phe, l-DOPA, l-Trp, serotonin , na ụfọdụ pyridyl-alkylamines bụ ndị na-arụ ọrụ ka mma, na K(A) s na nso 13-19 microM.Ndị na-arụ ọrụ CA VI kacha mma bụ l-Phe, d-DOPA, l-Tyr, 4-amino-l-Phe, na histamine, yana K (A) s na oke nke 1.23-9.31 microM.Ndị na-eme ihe ndị a niile na-eme ka k (cat), na-enweghị mmetụta na K (M), na-ekere òkè na ọnụego na-ekpebi nzọụkwụ na catalytic okirikiri, proton na-enyefe mmeghachi omume n'etiti saịtị na-arụ ọrụ enzyme na gburugburu ebe obibi.