L-Proline Cas: 147-85-3 99% ntụ ntụ ọcha
| Nọmba katalọgụ | XD90293 |
| Aha ngwaahịa | L-Proline |
| CAS | 147-85-3 |
| Usoro ihe omimi | C5H9NO2 |
| Ibu molekụla | 115.13046 |
| Nkọwa nchekwa | Ambient |
| Koodu tarifu akwadoro | 29339980 |
Nkọwapụta ngwaahịa
| Nyocha | 99% nkeji |
| Ọdịdị | Ọcha ntụ ntụ |
| Ntughari pụrụ iche | -84.5 ruo -86 |
| Ọla dị arọ | <15pm |
| AS | <1pm |
| Ph | 5.9-6.9 |
| SO4 | <0.050% |
| Fe | <30ppm |
| Ọnwụ na ihicha | <0.3% |
| Ihe fọdụrụ na mgbanye | <0.10% |
| NH4 | <0.02% |
| Cl | <0.050% |
| Ọnọdụ Ngwọta | > 98% |
Ịghọta metabolism nke microbial host dị oké mkpa maka mmepe na njikarịcha nke usoro biocatalytic dabeere na cell dum, n'ihi na ọ na-ekpebi nrụpụta mmepụta.Nke a bụ eziokwu karịsịa maka redox biocatalysis ebe a na-arụ ọrụ sel nke na-arụ ọrụ metabolic n'ihi ikike nke na-eme ka cofactor / cosubstrate na-eme ka ọ dị elu na onye ọbịa.A na-eji recombinant Escherichia coli mee ihe maka mmepụta proline-4-hydroxylase (P4H), dioxygenase na-eme ka hydroxylation nke L-proline n'efu n'ime trans-4-hydroxy-L-proline na a-ketoglutarate (a-KG) dị ka cosubstrate.Na biocatalyst nke cell a dum, Central carbon metabolism na-enye cosubstrate a-KG chọrọ, na-ejikọta P4H biocatalytic arụmọrụ ozugbo na carbon metabolism na metabolic ọrụ.Site n'itinye ma ngwaọrụ nnwale na mgbakọ na mwepụ, dị ka metabolic engineering na (13) C-metabolic flux analysis ((13)C-MFA), anyị nyochara na quantitatively kọwara physiological, metabolic, na bioenergetic nzaghachi nke dum-cell biocatalyst. na ntụgharị bioconversion nke ezubere iche wee chọpụta ihe mgbochi metabolic nwere ike ime maka injinia ụzọ ezi uche dị na ya. Ewubere ụdị mmebi proline enweghị ụkọ E. coli site na ihichapụ mkpụrụ ndụ putA na-edobe proline dehydrogenase.Mgbanwe mkpụrụ ndụ nke mkpụrụ ndụ niile nwere ụdị mutant a butere ọ bụghị naanị na quantitative proline hydroxylation kamakwa ọ na-abawanye okpukpu abụọ nke ụdị nhazi trans-4-L-hydroxyproline (hyp) akọwapụtara, ma e jiri ya tụnyere ụdị anụ ọhịa.Nyocha nke mgbagharị carbon site na metabolism nke etiti nke mutant mutant kpughere na mmụba a-KG maka ọrụ P4H emeghị ka a-KG na-emepụta flux, na-egosi ọrụ okirikiri TCA a na-achịkwa nke ọma n'okpuru ọnọdụ ndị a mụrụ.N'ụdị ụdị anụ ọhịa, njikọ P4H na catalysis kpatara mbelata mkpụrụ biomass.N'ụzọ na-akpali mmasị, eriri ΔputA na-akwụghachikwa ụgwọ ATP na NADH metụtara site n'ibelata mkpa ike na-arụ ọrụ na ọnụ ọgụgụ nnabata glucose dị ala, kama ịba ụba ọrụ TCA. The putA knockout in recombinant E. coli BL21(DE3)(pLysS) hụrụ ka na-ekwe nkwa maka catalysis P4H na-arụpụta ọ bụghị naanị n'ihe gbasara mkpụrụ ndụ biotransformation, kamakwa gbasara ọnụego maka biotransformation na nnabata proline na mkpụrụ nke hyp na isi iyi ike.Nsonaazụ na-egosi na, na ịkụ ọkpọ, njikọ nke TCA-cycle na proline hydroxylation site na cosubstrate a-KG na-aghọ isi ihe na-egbochi ya na ebumnuche iji meziwanye arụmọrụ nke a-KG-based biotransformations.
