Ngwaahịa

A na-eji spectroscopy infrared akọwapụta mmekọrịta nke mkpụrụ-protein: mgbanwe mgbanwe nke sarcoplasmic reticulum Ca (2+) -ATPase n'elu njikọ nucleotide na ATPase phosphorylation na-enyocha site na iji substrate ATP na ATP analogues (2'-deoxy-ATP, 3). '-deoxy-ATP, na inosine 5'-triphosphate), nke emegharịrị na otu ọrụ dị iche iche nke mkpụrụ.Mgbanwe na 2'-OH, 3'-OH, na amino group nke adenine na-ebelata oke mgbanwe mgbanwe mgbanwe nke ATPase na-ejikọta ya, na-enwe mmetụta siri ike na-ahụ maka abụọ ikpeazụ.Nke a na-egosipụta mmetụta nhazi nke nucleotide-ATPase mgbagwoju anya na mmekọrịta onye ọ bụla n'etiti nucleotide na ATPase.Otu niile a na-amụ dị mkpa maka ijikọ na mmekọrịta nke otu ligand nyere na ATPase na-adabere na mmekọrịta nke ndị ọzọ ligand.A hụrụ phosphorylation nke ATPase maka ITP na 2'-deoxy-ATP, mana ọ bụghị maka 3'-deoxy-ATP.Enweghị njikọ kpọmkwem n'etiti n'ókè nke mgbanwe mgbanwe na nucleotide na-ejikọta na ọnụ ọgụgụ nke phosphorylation na-egosi na njedebe zuru oke nke mgbanwe mgbanwe nke ATP na-eme ka ọ bụrụ iwu na phosphorylation.Dị ka a na-ahụ maka mgbagwoju anya nucleotide-ATPase, nhazi nke mbụ phosphorylated ATPase intermediate E1PCa (2) na-adaberekwa na nucleotide, na-egosi na ATPase na-ekwu na-enwechaghị nhazi nhazi karịa ka a tụrụ anya na mbụ.