Ngwaahịa

Adenosine bụ onye na-achịkwa ụda vaskụla nke endogenous.Akwụsị ọrụ a nke adenosine site na nnabata ya na metabolism site na mkpụrụ ndụ endothelial microvascular (MVEC).Onye na-ebufe ihe na-ebute ụzọ bụ ENT1 (nha nhata nucleoside transporter subtype 1).MVEC na-egosipụtakwa onye na-ebugharị nucleobase (ENBT1) nke na-etinye aka na cellular flux nke adenosine metabolites dị ka hypoxanthine.Mgbanwe na nke ọ bụla n'ime usoro njem ndị a ga-emetụta bioactivity nke adenosine na metabolism ya, gụnyere nhazi nke radicals free oxygen.MVEC dịpụrụ adịpụ site na akwara skeletal nke ENT1 (+/+) na ENT1 (-/-) ụmụ oke nwere nrụgide oxidative kpatara site na simulated ischemia/reperfusion ma ọ bụ menadione.A tụlere ihe omume ENT1 na ENBT1 na-arụ ọrụ dabere na zero-trans influx kinetics nke ihe ntinye aha redio.Enwere mbelata ọnụego nke ENBT1-mediated hypoxanthine nnabata site na ENT1(+/+) MVEC mesoro menadione ma ọ bụ mgbe ekpughere ọnọdụ ndị na-eme ka isc hemia/reperfusion.N'okwu abụọ ahụ, superoxide dismutase mimetic MnTMPyP belatara mfu nke ọrụ ENBT1, na-etinye superoxide radicals na nzaghachi.N'ụzọ dị iche, MVEC dịpụrụ adịpụ na ENT1 (-/-) ụmụ oke egosighi mbelata ọrụ ENBT1 na ọgwụgwọ na menadione ma ọ bụ simulated ischemia / reperfusion, mana ha nwere ọkwa dị elu nke ọrụ catalase metụtara ENT1 (+/+) MVEC.Ihe data ndị a na-egosi na ọrụ ENBT1 na-ebelata na MVEC na nzaghachi nke radical superoxide na-ejikọta ya na ischemia/reperfusion mmerụ.MVEC dịpụrụ adịpụ na ENT1 (-/-) ụmụ oke anaghị egosi mbelata a na ENBT1, ikekwe n'ihi ụbara ọrụ catalase.