Guanidine Thiocyanate Sulfate Cas: 593-84-0 99% White na-acha ọcha crystalline ntụ ntụ
| Nọmba katalọgụ | XD90199 |
| Aha ngwaahịa | Guanidine Thiocyanate sulfate |
| CAS | 593-84-0 |
| Usoro ihe omimi | C2H4N4S |
| Ibu molekụla | 116.145 |
| Nkọwa nchekwa | Ambient |
| Koodu tarifu akwadoro | 29299000 |
Nkọwapụta ngwaahịa
| Ebe mgbaze | 115-121 Deg C |
| Nyocha | > 99% |
| Cl | <1% |
| Ọdịdị | Uzuzu crystalline ọcha ruo na-acha ọcha |
| Mmiri KF | <0.5% |
Mycobacterium ụkwara nta nwere ike ịdịgide ruo ọtụtụ iri afọ n'ime mmadụ.Ụzọ nzaghachi siri ike gụnyere polyphosphate inorganic [poly (P)], nke a na-emepụta na hydrolyzed site na polyphosphate kinase (PPK) na exopolyphosphatase (PPX), n'otu n'otu, na-arụ ọrụ nchịkwa dị mkpa na nkwụsị nke nje.Anyị na-egosi ebe a na M. ụkwara nta poly(P) na-ejikọta nwa oge na mmachi uto bacillary.Anyị na-achọpụtakwa M. ụkwara nta Rv1026 dị ka akwụkwọ akụkọ exopolyphosphatase na hydrolytic ọrụ megide ogologo yinye poly(P).N'iji usoro okwu tetracycline-inducible na-akụtu okwu nke Rv1026 (ppx2), anyị chọpụtara na M. ụkwara nta poly (P) nchịkọta na-eduga n'ịdị nwayọọ na-eto eto ma na-ebelata ohere nke isoniazid, ụbara iguzogide okpomọkụ na acid pH, na nkwalite ndụ intracellular. n'oge ọrịa macrophage.Site na microscopy eletrọn na-ebufe, eriri ppx2 knockdown gosipụtara ọkpụrụkpụ mgbidi cell na-abawanye, nke jikọtara ya na mbelata mgbidi cell na-adaba na ọgwụ hydrophilic kama ịmalite nfuli nfuli ọgwụ ma ọ bụ nguzobe biofilm gbanwere n'ihe metụtara njikwa vector efu.Ntụle transcript na metabolomic gosipụtara mbelata nke metabolic nke ppx2 knockdown nke ejiri mbelata ederede na ntụgharị yana mbelata ọkwa glycerol-3-phosphate.Na nchịkọta, poly (P) na-arụ ọrụ dị mkpa na M. ụkwara nta nke ntachi obi na metabolic downshift ma na-enye aka na ntachi obi nke ọgwụ nje site na-agbanwe agbanwe mgbidi mgbidi. Nzaghachi siri ike, gụnyere ụmụ irighiri ihe nchịkwa nke inorganic polyphosphate [poly (P)] na (p). )ppGpp, ekwenyere na ọ na-emezi nkwụsi ike nke ụkwara nta Mycobacterium.N'ime ọmụmụ ihe a, anyị chọpụtara enzyme ọhụrụ (Rv1026, PPX2) maka hydrolyzing poly (P) ogologo yinye.A genetically engineered M. tuberculosis strain deficient na ppx2 gene gosipụtara mmụba nke poly(P), bụ nke jikọtara ya na njide uto nke nje bacteria n'oge na mbelata susce ptibility na ọgwụ isoniazid nke mbụ, yana ịba ụba nke nje bacteria n'oge ikpughe nrụgide. ọnọdụ na n'ime macrophages.N'ihe metụtara ụdị njikwa ahụ, mutant gosipụtara ụbara ọkpụrụkpụ nke mgbidi cell na mbelata ikike ọgwụ.Okwu mkpụrụ ndụ ihe nketa zuru ụwa ọnụ na nyocha metabolite gosipụtara mbelata ngosipụta nke igwe ederede na ntụgharị yana ngbanwe n'iji ikuku carbon eme ihe.Na nchịkọta, nhazi nke nguzozi nke poly(P) dị oke mkpa maka ịmepụta na-aga n'ihu na ụkwara nta M.
