Ngwaahịa

Otu n'ime ihe ndị na-egbochi ngwa ụlọ ọgwụ nke ọgwụ na-emegharị ahụ na ọrịa cartilage mebiri emebi bụ ebe kwesịrị ekwesị nke mkpụrụ ndụ.Chondrocytes, bụ nanị ụdị cell nke cartilage, toro na vitro n'okpuru ọnọdụ omenala iji gbasaa ọnụọgụ cell na-efunahụ phenotype ha yana ikike ịmepụta anụ ahụ cartilaginous hyaline.N'ime ọmụmụ ihe a, anyị na-ekpebi na usoro omenala nke serum- na uto-free-free three-dimensional (3D) omenala na-eweghachi ikike nke chondrocytes nke a na-agafe agafe na-emepụta anụ ahụ cartilage na vitro, usoro nke gụnyere sox9.Bovine articular chondrocytes na-agafe ugboro abụọ na-aga. nye ohere maka mgbasawanye nke nọmba cell (P2) ma mebe ya na njupụta dị elu na membranes mkpuchi 3D collagen-ụdị II na mgbasa ozi ọdịnaya glucose dị elu agbakwunyere na insulin na dexamethasone (SF3D).A mara mkpụrụ ndụ ndị ahụ ka mgbasawanye monolayer na ịgbaso omenala 3D site na cytometry eruba, okwu mkpụrụ ndụ ihe nketa, na akụkọ ihe mere eme.Mgbanwe ndị mbụ na ụzọ ntụgharị akara ngosi n'oge rediferentiat ion bụ ihe e ji mara. Mkpụrụ ndụ P2 gosipụtara profaịlụ antigen nke progenitor dị ka 99% CD44 (+) na 40% CD105 (+) na profaịlụ mkpụrụ ndụ ihe nketa na-egosi sel interzone.P2 na SF3D gosipụtara mkpụrụ ndụ ihe nketa chondrogenic yana akwakọba matrix extracellular.Mbelata insulin receptor (IR) na HNMPA- (AM3) ma ọ bụ ụzọ PI-3/AKT kinase (nke insulin na-arụ ọrụ) yana Wortmannin na-egbochi njikọ nke collagen.HNMPA- (AM3) belatara okwu nke Col2, Col11, na mkpụrụ ndụ IR yana Sox6 na -9.Nleba anya nyocha nke chromatin na chromatin immunoprecipitation nke HNMPA- (AM3) - mkpụrụ ndụ ndị a na-emeso gosipụtara njikọ nke coactivators Sox6 na Med12 na Sox9 ma belata Sox9-Col2a1 binding. Anyị na-akọwa usoro omenala ọhụrụ nke na-enye ohere ịbawanye ọnụ ọgụgụ nke chondrocytes ma na-akwalite. Nhazi anụ ahụ dị ka cartilage hyaline n'otu akụkụ site na njikọ insulin-mediated Sox9-Col2a1.Kwesịrị ịdị mma nke anụ ahụ emepụtara site na usoro a maka iji na-arụzi nkwonkwo kwesịrị ka enyocha ya na vivo.