Ngwaahịa

Protein ORF45 nke Kaposi's sarcoma-associated herpesvirus (KSHV) bụ protein tegument nke gammaherpes kpọmkwem kpọmkwem.Ọmụmụ ihe anyị gara aga ekpughere ọrụ ya dị oke mkpa n'oge mmalite na nke ikpeazụ nke ọrịa KSHV.N'ime ọmụmụ ihe a, anyị nyochara mmekọrịta nke ORF45 site na iji otu panel nke ọgwụ mgbochi monoclonal.Na mgbakwunye na nke achọpụtara na mbụ extracellular regulated kinase (ERK) na p90 ribosomal S6 kinase (RSK) proteins, anyị hụrụ ọtụtụ protein ndị ọzọ copurified, gụnyere ndị a ma ama nke ~ 38 kDa na ~ 130 kDa.Mass spectrometry kpughere na protein 38-kDa bụ viral ORF33 na protein 130-kDa bụ cellular USP7 (protease 7 kpọmkwem ubiquitin).Anyị debere ngalaba ORF33 na-echekwa carbon-terminal 19 amino acids (aa) nke ORF45 na ngalaba USP7 na ebumnuche nkwenye a kọrọ na mpaghara etiti ORF45.N'iji immunofluorescence staining, anyị hụrụ colocalization nke ORF45 na ORF33 ma ọ bụ USP7 ma n'okpuru transfected condit ion na na KSHV-ebutere sel.Ọzọkwa, anyị hụrụ ntugharị dabere na ORF45 nke akụkụ nke ORF33/USP7 site na nucleus gaa na cytoplasm.Anyị chọpụtara na ORF45 butere mmụba na mkpokọta protein ORF33 nke kwụsịrị ma ọ bụrụ na ehichapụrụ ORF33- ma ọ bụ USP7-binding ngalaba na ORF45.Ọzọkwa, ihichapụ ọdụ ụgbọ ala carboxyl echekwara nke ORF45 na genome KSHV belatara nke ukwuu ọkwa protein ORF33 n'ime mkpụrụ ndụ butere ọrịa KSHV yana kagburu mmepụta nke ụmụ virions.N'ịchịkọta, nsonaazụ anyị abụghị naanị ikpughe akụkụ ọhụrụ nke ORF45 interactome, kamakwa na-egosi na mmekọrịta dị n'etiti protein ndị a dị oke mkpa maka mmegharị nke KSHV lytic. Kaposi's sarcoma-associated herpesvirus (KSHV) bụ ihe na-akpata ọrịa ọtụtụ ọrịa cancer mmadụ.KSHV ORF45 bụ protein multifunctional nke achọrọ maka mmegharị nke KSHV lytic, mana usoro nke ORF45 ji arụ ọrụ ya dị oke mkpa amabeghị.Ọmụmụ ihe anyị gara aga gosiri na ORF45 pr otein niile dị n'ime sel dị n'ime ogige dị arọ dị elu.Ya mere, anyị chọrọ ịkọwapụta mmekọrịta nke ORF45 iji nye nkọwa gbasara ọrụ ya n'oge mmegharị lytic.N'iji otu panel nke ọgwụ mgbochi monoclonal, anyị nyochara ORF45 interactome na sel ndị butere ọrịa KSHV.Anyị chọpụtara ndị mmekọ ọhụrụ abụọ nke ORF45: protein viral ORF33 na cellular ubiquitin-specific protease 7 (USP7).Anyị na-egosipụta n'ihu na mmekọrịta dị n'etiti ORF45 na ORF33 dị oke mkpa maka mmepụta nke ọma nke nje virus KSHV, na-atụ aro na nnyonye anya ezubere iche na mmekọrịta a nwere ike ịnọchite anya atụmatụ ọhụrụ iji gbochie KSHV lytic replication.