Geneticin disulfate CAS: 108321-42-2 ntụ ntụ ọcha
| Nọmba katalọgụ | XD90373 |
| Aha ngwaahịa | Geneticin disulfate |
| CAS | 108321-42-2 |
| Usoro ihe omimi | C20H40N4O10 2H2SO4 |
| Ibu molekụla | 692.71 |
| Nkọwa nchekwa | 2 ruo 8 Celsius C |
| Koodu tarifu akwadoro | 29419000 |
Nkọwapụta ngwaahịa
| Nyocha | 99% |
| Ọdịdị | Ọcha ntụ ntụ |
| Maka iji nyocha naanị, ọ bụghị maka ojiji mmadụ | A na-eji nyocha naanị, ọ bụghị maka mmadụ |
Anyị mepụtara sistemu nnyefe RNA (siRNA) ọhụrụ nke na-eji ternary complex nwere polyethyleneimine (PEI) na γ-polyglutamic acid (γ-PGA), nke gosipụtara mmetụta ịgbachi nkịtị na enweghị cytotoxicity.Ọgbakọ ọnụọgụ abụọ nke siRNA nwere PEI dị ihe dịka 73-102 nm na nha nha yana 45-52 mV na ζ-nwere ike.Mmetụta ịgbachi nkịtị nke ogige siRNA/PEI mụbara site na mmụba nke PEI, yana ogige siRNA/PEI nwere oke ụgwọ karịrị 16 gosipụtara nnukwu luciferase knockdown n'ahịrị cell carcinoma mouse nke na-ekwupụta luciferase mgbe niile (Col26/Luc sel).Otú ọ dị, a hụrụ cytotoxicity siri ike na agglutination ọbara na mgbagwoju siRNA/Lipofectamine na siRNA/PEI16 complex.Akọkwara na ịjinye ogige cationic na ogige anionic bụ ụzọ na-ekwe nkwa maka imeri nsi ndị a.Ya mere, anyị ji PEI kwadoo ogige ternary nke siRNA site na mgbakwunye γ-PGA iji belata cytotoxicity ma wepụta siRNA.Dị ka a tụrụ anya, cytotoxicity nke ogige ternary belatara site na mmụba nke ọdịnaya γ-PGA, nke belatara ζ-ike nke ogige ahụ.Achọpụtara mmetụta ịgbachi nkịtị siri ike yiri mgbagwoju siRNA/Lipofectamine n'ime ụlọ ternary gụnyere γ-PGA nwere ụgwọ elu anionic.Ejikwa microcopy fluorescence kwadoro nnabata dị elu nke ogige ternary n'ime sel Colon26/Luc.N'ịbụ onye nwetasịrị ịkụda mkpụrụ ndụ ihe nketa na-agafe agafe, a tụlere ikike nke mgbagwoju anya ịgbagha mkpụrụ ndụ ihe nketa ụlọ, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), na sel B16-F10.Ihe mgbagwoju anya nke ternary (siRNA/PEI16/γ-PGA12 complex) gosipụtara mmetụta ịkụ aka GAPDH dị ịrịba ama.Ya mere, anyị mepụtara usoro nnyefe siRNA bara uru.
