Deoxyribonucleic acid, sperm sperm Cas: 100403-24-5
Nọmba katalọgụ | XD90579 |
Aha ngwaahịa | Deoxyribonucleic acid, sperm azụ |
CAS | 100403-24-5 |
Usoro ihe omimi | - |
Ibu molekụla | - |
Nkọwa nchekwa | 2 ruo 8 Celsius C |
Koodu tarifu akwadoro | 29349990 |
Nkọwapụta ngwaahịa
Ọdịdị | Ọcha ntụ ntụ |
Nyocha | 99% |
Pathogenic molecular ụzọ na cirrhotic ọrịa imeju dị ka ịba ọcha n'anya C (HCV), autoimmune ịba ọcha n'anya (AIH) na isi biliary cirrhosis (PBC) na-adịghị mma.Mkpụrụ ndụ ihe nketa dị iche iche na-adịkarị mkpa na pathogenesis ọrịa.Mgbochi subtractive hybridization (SSH) bụ usoro genome n'obosara na-eme ka ọ baa ụba maka transcript mRNA egosipụtara iche.Anyị bu n'obi ime nyocha ọhụụ nke mkpụrụ ndụ ihe nketa dị iche iche na cirrhosis site na iji SSH jikọtara yana quantitative real-time reverse transcriptase polymerase chain reaction (RT-PCR).SSH nyochara transcriptomes imeju na HCV cirrhosis, AIH cirrhosis, PBC, na anụ ahụ na-adịghị ọrịa.Ihe arụpụtara mmekọ DNA (cDNA) clones ka emegharịrị maka ngosipụta dị iche site na njiko ntụpọ ntụpọ wee hazie usoro.Agụpụtara mkpụrụ ndụ ihe nketa ahọpụtara site na RT-PCR ozugbo.Na-eso SSH, 694 clones ka emegharịrị maka ngosipụta mkpụrụ ndụ dị iche, nke 145 bụ usoro wee chọpụta na ha nwetara site na mkpụrụ ndụ ihe nketa 89 dị iche iche.clone asaa bụ otu n'ime naanị ya nwere usoro mkpado usoro egosipụtara (EST) na-etinye mkpụrụ ndụ ihe nketa na-enweghị ọrụ amaghi ama.E gosipụtara ngosipụta nke usoro mkpụrụ ndụ ihe nketa anọ site na RT-PCR dị adị: transmembrane 4 superfamily so 3 (tetraspanin CO-029) n'ụdị ọ bụla nke cirrhosis, protein mmekọrịta hedgehog (HIP) na AIH cirrhosis na chitinase 3-like-1 (HC gp-39 ma ọ bụ ykl-40) na arginine-glutamic acid ikwugharị (RERE) na HCV cirrhosis.RERE gene polymorphisms na splice variants ka ahụrụ n'anụ ahụ niile nyochara.Edebere ụkpụrụ nke Tetraspanin CO-029 na sel bile ductular.N'ikpeazụ, a na-eme nchọpụta ọhụrụ nke mkpụrụ ndụ ihe dị iche na cirrhosis mmadụ site na iji SSH dị ka ngwá ọrụ nchọpụta bụ isi.Karịsịa, nyocha ọzọ gbasara mkpụrụ ndụ RERE na ngwaahịa ya na ọrịa imeju metụtara HCV kwesịrị ekwesị.