dCTP, 2′-Deoxycytidine-5′-triphosphoric acid disodium nnu Cas:102783-51-7 White ntụ ntụ.
| Nọmba katalọgụ | XD90584 |
| Aha ngwaahịa | dCTP, 2'-Deoxycytidine-5'-triphosphoric acid disodium nnu |
| CAS | 951-78-0 |
| Usoro ihe omimi | C9H12N2O5 |
| Ibu molekụla | 228.20 |
| Nkọwa nchekwa | -20 Celsius |
| Koodu tarifu akwadoro | 29349990 |
Nkọwapụta ngwaahịa
| Ọdịdị | Ọcha ntụ ntụ |
| Nyocha | 99% |
Mkpụrụ ndụ Eukaryotic nwere nguzozi dị nro nke nkeji nke deoxyribonucleoside triphosphates (dNTPs), zuru ezu naanị nkeji ole na ole nke mmegharị DNA.Ma ụkọ na njupụta nke otu dNTP nwere ike ịkpata mmụba ngbanwe, nrụzi DNA na-ezighi ezi ma ọ bụ mmebi DNA mitochondrial.A na-agụkarị dNTP site na nyocha enzymatic nke ntinye nke redioactive dATP (ma ọ bụ dTTP redioactive na nyocha maka dATP) n'ime oligonucleotides sịntetik kpọmkwem site na DNA polymerase dabara na ntinye nke dNTP amaghi ama.Anyị na-achọpụta na Klenow DNA polymerase nke a na-ejikarị eme ihe nwere ike dochie ribonucleotide kwekọrọ maka dNTP amaghi ama na-eduga n'ụfọdụ na nnukwu nleba anya nke dNTP.Ugbu a anyị na-akọwa ọnọdụ nyocha maka dNTP ọ bụla na-ezere ntinye ribonucleotide.Maka nyocha dTTP na dATP ọ ga-ezuru iji belata mkpokọta Klenow enzyme na nke akpọrọ dATP (ma ọ bụ dTTP);maka dCTP na dGTP anyị ga-eji dochie T he Klenow enzyme ma ọ bụ Taq DNA polymerase ma ọ bụ Thermo Sequenase.Anyị na-atụ aro na n'ụfọdụ akụkọ ndị gara aga incorporation ribonucleotide nwere ike butere oke uru maka dGTP na dCTP.
