Ngwaahịa

Tet 5-methylcytosine dioxygenases na-eme ka DNA demethylation pụta site n'imepụta 5-hydroxymethylcytosine na ngwaahịa ndị ọzọ oxidized.A na-egosipụta Tet1 na Tet2 nke ukwuu n'ime sel pluripotent òké wee gbadaa ruo n'ókè dị iche iche na sel somatic, mana usoro ederede amabeghị.N'ebe a, anyị kọwapụtara ngalaba nkwalite na nkwalite na Tet1 na Tet2.N'ime 15-kb "superenhancer" nke Tet1, enwere saịtị mmalite ederede abụọ (TSS) nwere usoro mmalite dị iche iche n'oge mmepe.Mpaghara mgbasa ozi 6-kb dị elu nke TSS dị anya na-arụsi ọrụ ike na sel ndị na-enweghị isi, na-akọkwa okwu Tet1 n'onwe ya na sistemu transgenic, ma na-enweta DNA methylation ngwa ngwa na ịgbachi nkịtị n'iche dị na sel mepere emepe na epiblast ala.TSS nke abụọ na mgbada, jikọtara ya na onye nkwalite bara ụba nke CpG na-adịghị ike, bụ onye nkwalite agbata obi na sel embryonic stem cell (ESCs) na mkpụrụ ndụ epiblast dị ka EpiLC na-arụ ọrụ.Tet2 nwere CpG agwaetiti p romoter nwere ọrụ nwere onwe ya na pluripotency yana nkwalite intragenic dịpụrụ adịpụ nke ESC;A na-agbada nke ikpeazụ ngwa ngwa na EpiLCs.Ọmụmụ ihe anyị na-ekpughe ụdị dị iche iche nke ụkpụrụ ntụgharị na Tet1 na Tet2 n'oge mgbanwe steeti steeti nke mmalite mmalite.Ndị nta akụkọ transgenic ọhụrụ na-eji ngalaba Tet1 na Tet2 cis-regulatory nwere ike na-enyere aka ịmata mgbanwe mgbanwe dị iche iche na steeti ndị nwere ike ịdị iche yana ọdịiche epigenetic dị n'okpuru.