Ngwaahịa

CTP: phosphocholine cytidylyltransferase α (CCTα) bụ enzyme nuklia nke na-eme ka usoro nkwụsị ọnụego dị na ụzọ CDP-choline maka njikọ phosphatidylcholine (PC).Nkwalite lipid nke CCTα na-ebute ntụgharị ya na envelopu nuklia na mgbasawanye nke netwọk akpụkpọ anụ intranuclear a na-akpọ nucleoplasmic reticulum (NR) site na usoro metụtara nrụrụ akpụkpọ ahụ.A na-achọkwa lamins nuklia maka nkwụsi ike na ịba ụba nke NR, mana ma a chọrọ usoro a pụrụ iche, ma ọ bụ lamina nuklia n'ozuzu, maka njikọ PC adịghị ama.Iji nyochaa mmekọrịta a, RNAi mebiri lamina nuklia ma ọ bụ mebie ya site na nkwupụta nke Hutchinson-Gilford progeria syndrome (HGPS) mutant lamin A (progerin), na mmetụta na CCTa na choline metabolism na-enyocha.siRNA gbachitere ịgbachi nkịtị nke lamin A/C ma ọ bụ lamin B1 n'ime mkpụrụ ndụ CHO iji belata NR enweghị mmetụta na njikọ PC, ebe ịkụda okpukpu abụọ na-egbochighị ụzọ ahụ.N'ịkwado na obere ọrụ ya na njikọ PC, naanị 10% nke choline/ethanolamine phosphotransferase na-agafe agafe ka achọpụtara na NR.N'ime mkpụrụ ndụ CHO, CCTa bụ nucleoplasmic ma jikọta ya na GFP-progerin na nkpuchi nuklia na invaginations;Otú ọ dị, HGPS fibroblasts gosipụtara nkesa na-adịghị mma nke CCTα na cytoplasm na envelopu nuklia nke jikọtara ya na mbelata okpukpu abụọ na njikọ PC.N'agbanyeghị mgbanwe ya na mpaghara ya, nchọpụta choline-labeling gosiri na ọrụ CCT adịghị emetụta ya, na mgbochi nke PC synthesis bụ nke a na-achọpụta na mbelata ọrụ nke onye na-ebugharị choline na-enwe mmetụta hemicholinium.Anyị kwubiri na CCTα na lamins na-arụkọ ọrụ ọnụ iji mepụta NR, mana nhazi nke envelopu nuklia nwere ntakịrị mmetụta na ọrụ CCT na njikọ PC.