Ngwaahịa

Ndị brucellae bụ ndị na-ahụ maka etiological nke brucellosis, zoonosis ekesara n'ụwa nile.Nje bacteria ndị a bụ nje nje ndị na-akpata intracellular na-arụ ọrụ ma si otú a na-enwe ike ịhazigharị metabolism ha na gburugburu mpụta na intracellular ha na-ezute n'oge nje na-efe efe.Agbanyeghị, akụkụ a nke bayoloji Brucella ka aghọtaghị nke ọma, amabeghịkwa nri dị na niche intracellular amabeghị.N'ebe a, anyị nyochara ụzọ etiti C metabolism nke Brucella abortus ji mee ihe site na ihichapụ putative fructose-1,6-bisphosphatase (fbp na glpX), phosphoenolpyruvate carboxykinase (pckA), pyruvate phosphate dikinase (ppdK), na malic enzyme (mae). mkpụrụ ndụ ihe nketa.Na gluconeogenic ma ọ bụghị na mgbasa ozi bara ụba, uto nke ΔppdK na Δmae mutants nwere nnukwu nsogbu na uto nke okpukpu abụọ Δfbp-ΔglpX mutant belatara.Na macrophages, naanị ΔppdK na Δmae mutant gosipụtara mbelata ịba ụba, na ọmụmụ ihe na ΔppdK mutant kwadoro na ọ ruru niche oyiri.N'otu aka ahụ, naanị ΔppdK na Δmae mutants na-ebelata na ụmụ oke, nke mbụ na-ekpochapụ site na izu 10 na nke ikpeazụ na-adịgide karịa izu 12.Anyị nyochakwara okirikiri glyoxylate.Ọ bụ ezie na ọrụ nkwalite aceA (isocitrate lyase) kwalitere n'ọgaranya ọgaranya, nkwụsịtụ aceA enweghị mmetụta na vitro ma ọ bụ ịba ụba na macrophages ma ọ bụ spleens òké.Nsonaazụ na-egosi na B. abortus na-eto intracellularly site na iji obere ọkọnọ nke 6-C (na 5-C) shuga na-akwụ ụgwọ site na glutamate na ikekwe amino acid ndị ọzọ na-abanye na okirikiri Krebs na-enweghị ọrụ dị oke egwu nke glyoxylate shunt.