Ngwaahịa

Ejiri iji mepụta ihe nchekwa CAPS, ihe nchekwa zwitterionic bara uru na oke pH 7.9-11.1.A na-eji ihe nchekwa CAPS eme ihe n'ọtụtụ ebe na nyocha nke ọdịda anyanwụ yana nyocha immunoblotting yana usoro protein na njirimara.A na-eji ya na nnyefe electro nke protein na PVDF (sc-3723) ma ọ bụ akpụkpọ anụ nitrocellulose (sc-3718, sc-3724).PH dị elu nke ihe nchekwa a na-eme ka ọ baa uru maka ịnyefe protein na pI> 8.5.na obere mmeghachi omume na enzymes ma ọ bụ protein, mmetụta nnu pere mpe.

Na mpaghara capillary, electrophoresis, ike electrophoretic nke ion na-ebelata ka ntinye nke ngwọta electrolyte ndabere na-abawanye.Nke a na-akpata site na mgbanwe na mmegharị electrophoretic nke ion (muep) yana mgbanwe na ike net na-emetụta ya, ya bụ ike eletrik dị irè (Eeff).A na-agbanwegharị agagharị electrophoretic nke ion site na mgbanwe na viscosity zuru oke nke ngwọta electrolyte na mgbanwe na nha nke ion edoziri.A na-agbanwe Eeff tumadi site na mgbanwe n'ịdị ukwuu nke mmetụta asymmetry chaja na mmetụta electrophoretic, nke abụọ na-akwụsị mmegharị nke ion.N'ime ọmụmụ ihe a, a na-eji usoro akara atọ mee ihe iji mụọ mmetụta nke ndabere electrolyte fojusi (0.02-0.08M 3-[cyclohexylamino] -1-propanesulfonic acid na counter ion (Li, Na, K, na Cs) na Eeff. Achọpụtara na ntinye nke electrolyte n'azụ na-emetụta Eeff nke ọma na Eeff na-abịarute E ka ntinye nke electrolyte ndabere na-abịaru nso efu. ion counter nwere obere mmetụta na Eeff: dịka nha nke radius hydrated nke counter ion na-abawanye. Usoro akara atọ gosipụtara na ọ dị mma maka mkpebi ndị dị otú ahụ.