Ngwaahịa

Ọrụ ndu: BCIP (BCIP p-toluidine nnu; nnu X-phosphate p-toluidine) bụ mkpụrụ chromogenic maka nchọpụta colorimetric nke ọrụ alkaline phosphatase.

A na-emepụta nsị Shiga (Stxs) site na enterohemorrhagic Escherichia coli (EHEC), nke na-ebute ọrịa mmadụ na-ebutekarị ọnwụ.Ahịrị mkpụrụ ndụ Vero, nke sitere na akụrụ enwe ndụ ndụ ndụ nke Africa, na-anọchi anya ọkọlọtọ ọla edo maka ịchọpụta mmetụta cytotoxic nke Stxs.N'agbanyeghị ojiji ha zuru ụwa ọnụ, ihe ọmụma banyere kpọmkwem ihe owuwu nke Stx receptor glycosphingolipids (GSLs) na mgbakọ ha na lipid rafts adịghị mma.N'ebe a, anyị na-egosi nyocha nhazi zuru oke nke Stx receptor GSLs na nkesa ha na akpụkpọ anụ na-eguzogide ọgwụ (DRMs), nke a kwadebere site na mkpụrụ ndụ Vero-B4 ma jiri ya mee ihe dị ka lipid raft.Anyị chọpụtara globotriaosylceramide (Gb3Cer) na globotetraosylceramide (Gb4Cer) dị ka ndị nnabata GSL maka ụdị Stx1a, Stx2a, na Stx2e site na iji nchọpụta ihe mkpuchi TLC jikọtara yana MS.Onye na-anabata Stx na-adịghị ahụkebe, globopentaosylceramide (Gb5Cer, Galβ3GalNAcβ3Galα4Galβ4Glcβ1Cer), nke Stx2e ghọtara nke ọma (na mgbakwunye na Gb3Cer na Gb4Cer) site na Stx2e, bụ nke zuru oke.Achọpụtara lipoforms nke Stx receptor GSL na-abụkarị ọdụ ụgbọ mmiri ceramide mejupụtara sphingosine (d18:1) na C24: 0/C24: 1 ma ọ bụ C16: 0 fatty acid.Ọzọkwa, njikọta na ihe nrịbama lipid raft, SM na cholesterol, na DRM tụrụ aro njikọ GSL na microdomains membrane.Ọmụmụ ihe a na-enye ihe ndabere maka ịga n'ihu na-enyocha mmetụta arụ ọrụ nke ndị na-anabata Stx na-ejikọta lipid raft maka mmerụ ahụ na-egbu egbu nke mkpụrụ ndụ Vero-B4.