Ngwaahịa

Reagents nke Trinder ọhụrụ bụ ihe analine na-agbaze mmiri nke ukwuu nke a na-ejikarị na nyocha nyocha na nyocha biochemical.Enwere ọtụtụ uru karịa reagents chromogenic nkịtị na mkpebi colorimetric nke ọrụ hydrogen peroxide.Ihe reagents nke Trinder ọhụrụ kwụsiri ike nke ọma ka ejiri ya mee ihe na usoro nyocha na nyocha pipeline.N'ebe ọnụnọ nke hydrogen peroxide na peroxidase, akwụkwọ akụkọ Trinder's reagent gosipụtara ka ọ na-emeghachi omume na 4-aminoantipyrine (4-AA) ma ọ bụ 3- methylbenzothiazole sulfonehydrazone (MBTH) n'oge mmeghachi omume njikọ oxidative.Na-etolite ezigbo violet ma ọ bụ acha anụnụ anụnụ.Mpempe mkpọ nke agba nke jikọtara ya na MBTH bụ 1.5- 2 ugboro dị elu karịa nke agba agba yana 4-AA;Otú ọ dị, ngwọta 4-AA kwụsiri ike karịa ngwọta MBTH.A na-eme ka mkpụrụ ahụ na-eme ihe na-ekpo ọkụ site na oxidase ya iji mepụta hydrogen peroxide.Ntinye uche hydrogen peroxide kwekọrọ na ntinye mkpụrụ.Ya mere, ọnụọgụ nke mkpụrụ nwere ike ikpebi site na mmepe agba nke mmeghachi omume njikọ oxidative.Enwere ike iji glucose, mmanya na-aba n'anya, acyl-CoA na cholesterol iji chọpụta ihe ndị ahụ jikọtara ya na Trinder's reagent na 4-AA.Enwere reagents 10 ọhụrụ Trinder dị.N'ime ihe nrụpụta Trinder ọhụrụ, TOOS bụ nke a na-ejikarị.Agbanyeghị, maka otu mkpụrụ, ịnwale klaasị dị iche iche nke reagents nke Trinder dị mkpa iji mepụta sistemu nchọpụta kacha mma.