Ngwaahịa

Nleba anya nke pharmacodynamics na mgbakwunye na pharmacokinetics bụ otu n'ime atụmatụ iji wepụta ọgwụgwọ mycophenolate mofetil n'otu n'otu.Ebumnuche nke ọmụmụ a bụ ịzụlite mmetụta chromatography-tandem mass spectrometry (LC-MS/MS) ụzọ maka nyocha nke pharmacokinetics na pharmacodynamics nke mycophenolic acid (MPA).Achọpụtara ntinye nke mycophenolic acid glucuronide (MPAG), mycophenolic acid acyl-glucuronide, yana MPA na MPAG na-adịghị agwụ agwụ, na-agbakọ ọrụ inosine-5'-monophosphate dehydrogenase site n'ịleta mkpokọta xanthosine-5'-monophosphate (XMP). ) na intracellular adenosine-5'-monophosphate mgbe incubation nke mpụta ọbara mononuclear cell (PBMC) lysates.Ejiri kọlụm Mastro(TM) na-enweghị ọla yana ụkpụrụ gradient abụọ iji kwalite oke nha nke XMP ruo 0.1 μM.N'ime oke ịta ahụhụ MPA ụlọ ọgwụ, akara ahịrị nke usoro nhazigharị, nzizi n'etiti na n'ime ụbọchị na izi ezi na-ejupụta ntuziaka nchịkwa nri na ọgwụ US dị mkpa.Ntụle MPA na ndị ọrịa hematopoietic stem cell transplant (HSCT) kpebisiri ike site na nyocha enzyme na usoro LC-MS / MS dị ugbu a gosipụtara mmekọrịta dị mma (r (2) = 0.95, p <0.001).N'ime ọmụmụ ihe a, anyị na-akọ usoro LC-MS/MS dị nro na nke kwadoro iji nyochaa pharmacokinetics na pharmacodynamics nke MPA, nke na-enwe mmetụta zuru oke iji nyochaa obere ọnụọgụ PBMC anakọtara obere oge ka HSCT gasịrị.