Ngwaahịa

Ọdịdị 3D a na-ahụ anya nke DNA nwere eriri abụọ na-eme ka ọ dabara nke ọma dị ka ndebiri maka nhazi ala elu nke saịtị arụ ọrụ nucleic acid na-arụ ọrụ.N'ebe a, anyị na-enyocha iji DNA duplex 14mer dị ka ihe nhụsianya maka nhazi ziri ezi na nke a ga-ebu amụma maka ọrụ catalytic.Nyere nsonye zuru oke nke otu imidazole nke dabeere na histidine na njirimara protein na ihe omume catalysis, otu histidine dị ka duplexes gbanwere.Ijikọ histamine na C5 nke thymine base site na njikọ amide, na-enye ohere ngbanwe nke ọrụ imidazole na nnukwu oghere.A na-enyocha mmekọrịta dị n'etiti imidazole na duplex na mmetụta ya na imidazolium pKaH site n'itinye otu thymine gbanwetụrụ n'ọnọdụ anọ dị iche iche n'etiti 14mer helix abụọ.N'iji NMR na ihe mgbagwoju anya molecular na-achịkwaghị achịkwa, ihe nhazi nhazi nke gụnyere nhazi nke njikọ hydrogen n'etiti imidazole na Hoogste n'akụkụ akụkụ guanine nke ụzọ abụọ GC gbara agbata obi.Ebumnuche ahụ na-enye aka na nkwụsi ike megide mgbaze ọkụ nke 6 Celsius ma bụ isi n'ịgbanwe pKaH nke otu imidazolium.Akọwapụtara atụmatụ izugbe, ihe ndị achọrọ na njirimara nke ụdị pKaH ọhụrụ a na-achịkwa.