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4-Nitrophenyl Beta-D-glucuronide CAS: 10344-94-2 Ìhè Yellow ruo White ntụ ntụ 98%

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Nọmba katalọgụ: XD90021
CAS: 10344-94-2
Ụdị molekụla: C12H13NO9
Ibu Molecular: 315.23
Nnweta: Dị na ngwaahịa
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Nkwakọ ngwaahịa: 1g USD20
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Mkpado ngwaahịa

Nọmba katalọgụ XD90021
Aha ngwaahịa 4-Nitrophenyl Beta-D-glucuronide
CAS 10344-94-2
Usoro ihe omimi C12H13NO9
Ibu molekụla 315.23
Nkọwa nchekwa -15 ruo -20 Celsius
Koodu tarifu akwadoro 29329900

Nkọwapụta ngwaahịa

Ịdị ọcha (HPLC) ≥98%
Ọdịdị Ìhè edo edo ruo ọcha ntụ ntụ
HNMP Dabara n'usoro
Kart Fischer kacha 10%

Mkpụrụ ndụ beta-glucosidase (bgl3) sitere na Streptomyces sp.QM-B814 (Ụdị Ụdị Omenala America 11238) ka agbachiela site na mmeju ọrụ nke beta-glucosidase-negative mutant nke Streptomyces lividans.Achọpụtara oghere ọgụgụ mepere emepe nke 1440 nucleotides na-etinye koodu polypeptide nke amino acid 479 site n'usoro.Protein ahụ etinyere ya (Bgl3) na-egosi myirịta dị ukwuu (karịa njirimara 45%) yana beta-glycosidases sitere na ezinụlọ-1 glycosyl hydrolases.Enzyme cloned, sachara na-eso mmiri ozuzo ammonium sulphate na usoro chromatographic abụọ, bụ monomeric nwere oke molekụla 52.6 kDa, dịka ekpebie site na spectrometry mass, yana ebe isoelectric nke pI 4.4.Enzyme ahụ dị ka beta-glucosidase nwere nkọwapụta mkpụrụ osisi sara mbara, na-arụ ọrụ na celloligomer, ma na-eme mmeghachi omume transglycosylation.Atụmatụ Km pụtara ìhè maka p-nitrophenyl-beta-D-glucopyranoside na cellobiose bụ 0.27 mM na 7.9 mM, n'otu n'otu.Uru Ki maka glucose na delta-gluconolactone, na-eji p-nitrophenyl-beta-D-glucopyranoside dị ka mkpụrụ, bụ 65 mM na 0.08 mM, n'otu n'otu.Enzyme dị ọcha nwere pH kacha nke pH 6.5 na okpomọkụ kachasị maka ọrụ bụ ogo 50.


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    4-Nitrophenyl Beta-D-glucuronide CAS: 10344-94-2 Ìhè Yellow ruo White ntụ ntụ 98%