Ngwaahịa

Otu esi ejikọta mmetụta nke ngbanwe n'otu oge iji gbanwee ọrụ enzymatic adịghị mfe ịkọ n'ihi na mmetụta ndị a anaghị agbakwunye mgbe niile n'ụzọ ziri ezi.N'ihi ya, njirimara nke mmetụta nke mmụgharị nke amino acid n'otu oge nke na-ejikọta mkpụrụ nwere ike inye aka dị ukwuu n'ịghọta kpọmkwem mkpụrụ nke enzymes.Na beta-glycosidase sitere na Spodoptera frugiperda (Sfbetagly), ihe fọdụrụ Q39 na E451 na-emekọ ihe na mkpụrụ osisi na nke a dị mkpa maka ịkọwapụta kpọmkwem mkpụrụ.Mmụba nke Sfbetagly ugboro abụọ (A451E39, S451E39 na S451N39) bụ nke mutagenesis na-eduzi saịtị kwadoro, gosipụtara na nje bacteria wee sachaa site na iji chromatography mmekọrịta.Eji p-nitrophenyl beta-galactoside na p-nitrophenyl beta-fucoside ji mara enzymes ndị a dị ka mkpụrụ.A na-eji k cat/km ruru maka otu na mutant abụọ nke Sfbetagly nwere mmụgharị na-eduzi saịtị na ọnọdụ Q39 na E451 iji gosipụta na mmetụta na ike efu nke ESdouble dagger (enzyme-transition state complex) nke mgbanwe abụọ (Gdouble). daggerxy) abụghị nchikota mmetụta sitere na otu mmụgharị (Gdouble daggerx na Gdouble daggery).Ọdịiche a dị na Gdouble dagger na-egosi na mmetụta nke otu mmụgharị ahụ na-adaba n'otu akụkụ.N'ihi ya, mmetụta a na-ahụkarị na-agụ naanị otu ugboro na Gdouble daggerxy.Data Crystallographic na beta-glycosidases na-ekpughe ọnụnọ nke njikọ hydrogen bidentate gụnyere ihe fọdụrụ Q39 na E451 yana otu hydroxyl otu nke mkpụrụ.Ya mere, ma data thermodynamic na crystallographic na-egosi na ihe fọdụrụ Q39 na E451 na-enwe mmetụta n'otu n'otu na mmekọrịta ha na mkpụrụ osisi.