4-NITROPHENYL-ALPHA-D-MANNOPYRANOSIDE CAS:10357-27-4 Gbanyụọ-Ọcha ntụ ntụ 98%
| Nọmba katalọgụ | XD90011 |
| Aha ngwaahịa | 4-Nitrophenyl-alpha-D-mannopyranoside |
| CAS | 10357-27-4 |
| Usoro ihe omimi | C12H15NO8 |
| Ibu molekụla | 30301.25 |
| Nkọwa nchekwa | -2na-8Celsius C |
| Koodu tarifu akwadoro | 29400000 |
Nkọwapụta ngwaahịa
| Mmiri | <5% Karl Fische |
| Solubility | 1% na DMF doro anya na enweghị agba |
| Ịdị ọcha | 4-Nitrophenol efu <200ppm |
| HPLC | > 98% |
| Ọdịdị | Gbanyụọ-ọcha ntụ ntụ |
Nghọta usoro n'ime ezinụlọ Ca2+ dabere na alpha-mannosidases na eriri afọ mmadụ.
Bacteroides thetaiotaomicron gosipụtara, na-arụ ọrụ dị mkpa n'ịkwado ahụike mmadụ site n'iji ezinụlọ buru ibu nke glycoside hydrolases (GHs) na-erigbu polysaccharides nri na ndị ọbịa glycans dị ka nri.Mgbasawanye ezinụlọ GH dị otú ahụ bụ ihe atụ site na ezinụlọ 23 GH92 glycosidases nke B. thetaiotaomicron genome debere.N'ebe a, anyị na-egosi na ndị a bụ alfa-mannosidases na-eme site na otu usoro mbugharị iji jiri N-glycans ndị ọbịa.Ọdịdị akụkụ atọ nke GH92 mannosidases abụọ na-akọwa ezinụlọ nke protein ngalaba abụọ nke ebe catalytic center dị na interface ngalaba, na-enye enyemaka acid (glutamate) na isi (aspartate) na hydrolysis na Ca (2+) - usoro dabere.Ọdịdị akụkụ atọ nke GH92s dị mgbagwoju anya nwere ndị na-egbochi ya na-enye nghọta na nkọwapụta, usoro na usoro nhazi nke catalysis.Ca (2+) na-arụ ọrụ dị mkpa na-enyere aka ịgbagọ mannoside pụọ na ala ala ya (4)C (1) nhazi oche maka ọnọdụ mgbanwe. (Akwụkwọ akụkọ: Nat.Chem.Biol.6, 125-32, (2010)
chromatography nke ihu ihu nke ovalbumin glycoasparagines na kọlụm concanavalin A-sepharose.Ọmụmụ ihe ọnụọgụ nke njide kpọmkwem nke lectin.
Mmekọrịta nke Sepharose 4B-immobilized concanavalin A (ConA) na 10 glycoasparagines ewepụtara na ovalbumin ka a na-enyocha ọnụ ọgụgụ site na chromatography ihu ihu.Na usoro a, a na-etinye ngwọta carbohydrate na-aga n'ihu na kọlụm ConA-Sepharose na nkwụghachi azụ nke elution n'ihu ka a na-atụle dị ka oke nke ike nke mmekọrịta.Enwere ike ikpebi mgbe niile (Kd) maka saccharide ọ bụla nwere ConA.Nyocha nke njikọ nke p-nitrophenyl-alpha,D-mannoside egosila na ihe ejikọtara nke ConA anaghị agbanwe agbanwe mgbe emechara ya na Sepharose 4B.Nke ọ bụla n'ime ovalbumin glycoasparagines ka a na-akpọ tritium site na usoro methylation reductive maka nyocha.Ntụle nke ụkpụrụ Kd enwetara gosiri na njide nke ConA na-adịgasị iche n'iche dị iche iche n'usoro nhazi nke yinye glycosyl.Nsonaazụ na-egosi na ConA na-amata usoro nhazi glycosyl kpọmkwem, Man alpha 1-6 (Man alpha 1-3) Man, nke ma ọ dịkarịa ala otu hydroxyl na ọnọdụ C-3 nke mannose njikọ C-6 kwesịrị ịbụ n'efu.
